Detailed information of TA system TA232826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 39446..40182
• Replicon: plasmid unnamed2
• Accession: NZ_CP091127
• Organism: Serratia marcescens strain CM2015_854

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: L2D47_RS28505
• Protein ID: WP_003026803.1
• Coordinates: 39700..40182 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: L2D47_RS28500
• Protein ID: WP_003026799.1
• Coordinates: 39446..39712 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D47_RS28475 (L2D47_28470)	34609..35589	+	981	WP_014386491.1	IS5-like element ISKpn26 family transposase	-
L2D47_RS29460	35923..36045	-	123	WP_254913144.1	hypothetical protein	-
L2D47_RS28480 (L2D47_28475)	36145..36495	-	351	WP_004200891.1	hypothetical protein	-
L2D47_RS28490 (L2D47_28485)	38172..38720	+	549	WP_001567366.1	thioredoxin fold domain-containing protein	-
L2D47_RS28495 (L2D47_28490)	38767..39201	+	435	WP_001567367.1	cell envelope integrity protein TolA	-
L2D47_RS28500 (L2D47_28495)	39446..39712	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
L2D47_RS28505 (L2D47_28500)	39700..40182	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
L2D47_RS28510 (L2D47_28505)	40383..41786	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
L2D47_RS28515 (L2D47_28510)	41815..42447	-	633	WP_001567369.1	hypothetical protein	-
L2D47_RS28520 (L2D47_28515)	42971..43975	-	1005	WP_000427620.1	IS110-like element IS4321 family transposase	-
L2D47_RS28525 (L2D47_28520)	44054..44611	-	558	WP_003100847.1	recombinase family protein	-
L2D47_RS28530 (L2D47_28525)	44605..44976	-	372	WP_003100853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..193603	193603
	-	inside	IScluster/Tn	-	-	34609..51296	16687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T232826 WP_003026803.1 NZ_CP091127:39700-40182 [Serratia marcescens]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T232826 NZ_CP128260:2068437-2068649 [Pseudomonas fluorescens]
GTGCAGAGCAGGCTATTGATCAAGGAGCTGGAGGAAGCAGGCTGGACGCTGGACCGGATAACCGGCAGCCACCACATCTT
CACGCACCGTTACAACCCATACACGATTCCCGTCCCTCATCCGAAAAAGGATTTGCCGGTCGGGACGGTCAAGAGCATCA
GGAGGCGTGCCGGGTTGTACAACCCGCCAGCCAGTTACAGAGGAGATCCGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT232826 WP_003026799.1 NZ_CP091127:39446-39712 [Serratia marcescens]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT232826 NZ_CP128260:2068649-2069053 [Pseudomonas fluorescens]
ATGCAATACCCAATCTGCATCGAGTGGGGAGACGAGAACACCGCTATCGGGATTCAGATCCCCGATATTCCCGGCGCGGT
AACGGCCGGGGATACGTTTGAGGATGCTTACAACGCGGCCGTCGAGATTGCCCACATCATGCTGCAGGAGATCGCGGCGG
ATGGTGATTCGATTCCGATGCCGACTTCTGCGGCTGCACATCGCAACAACCCGGAGTTCGCGGACATGGGTTGGGGCATG
CTCGAGCTGGACATCTCGCCGTACATGGGCAAGACCGAAAAGGTCAATGTGACGTTGCCCGGCTATGTCATCCAGCGGAT
CGACCGCTACGTGCGTGAGCACAACGTCAAAAGCCGCTCCTCTTTCCTGGCGGATGCCGCCATGGAAAAACTGGTTCGGC
ATTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66