Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 10502..11027 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP091127 | ||
| Organism | Serratia marcescens strain CM2015_854 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | J5VBT8 |
| Locus tag | L2D47_RS28355 | Protein ID | WP_004197633.1 |
| Coordinates | 10722..11027 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | A0A1D8K3R4 |
| Locus tag | L2D47_RS28350 | Protein ID | WP_006788213.1 |
| Coordinates | 10502..10720 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L2D47_RS28325 (L2D47_28320) | 6628..7650 | - | 1023 | WP_151523148.1 | DNA helicase UvrD | - |
| L2D47_RS28330 (L2D47_28325) | 7635..9197 | - | 1563 | WP_004206609.1 | AAA family ATPase | - |
| L2D47_RS28335 (L2D47_28330) | 9271..9687 | - | 417 | WP_240285455.1 | type II toxin-antitoxin system VapC family toxin | - |
| L2D47_RS28340 (L2D47_28335) | 9684..9914 | - | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| L2D47_RS28345 (L2D47_28340) | 9871..10332 | + | 462 | WP_192802327.1 | hypothetical protein | - |
| L2D47_RS28350 (L2D47_28345) | 10502..10720 | + | 219 | WP_006788213.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| L2D47_RS28355 (L2D47_28350) | 10722..11027 | + | 306 | WP_004197633.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| L2D47_RS28360 (L2D47_28355) | 11085..11396 | + | 312 | WP_009309919.1 | hypothetical protein | - |
| L2D47_RS28365 (L2D47_28360) | 11446..11781 | + | 336 | WP_009309920.1 | hypothetical protein | - |
| L2D47_RS28370 (L2D47_28365) | 11815..12831 | + | 1017 | WP_009309921.1 | hypothetical protein | - |
| L2D47_RS28375 (L2D47_28370) | 13029..13808 | + | 780 | WP_023287113.1 | site-specific integrase | - |
| L2D47_RS28380 (L2D47_28375) | 13866..14123 | - | 258 | WP_009310077.1 | hypothetical protein | - |
| L2D47_RS28385 (L2D47_28380) | 14252..14365 | - | 114 | WP_014343462.1 | hypothetical protein | - |
| L2D47_RS28390 (L2D47_28385) | 14761..15741 | + | 981 | WP_014386491.1 | IS5-like element ISKpn26 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..193603 | 193603 | |
| - | flank | IS/Tn | - | - | 14761..15741 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11585.27 Da Isoelectric Point: 6.4661
>T232825 WP_004197633.1 NZ_CP091127:10722-11027 [Serratia marcescens]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T232825 NZ_CP128260:c2066746-2066243 [Pseudomonas fluorescens]
ATGTGGCTTGCCCTCTCGGGGCGATTTCGGGCGCGATGGACCCAGGAGATTCACTGCGAATGGAAACGCAATCTGCTGAA
GAACCGCCCTGACCTGACGATGGAACAGCTGGATCGTACGTCCGCCCTGATGGATCAAGCGATACCCGACGCCTGTTTCC
ATGACTATGAAGACCTGATTGCCGGACTCAGCTTGCCCGACTGCGATGATCGTCATGTTCTGGCAGCGGCCATCCGTTGC
GGCGCAAGCGTGATAGTGACGTTCAACCAGAAAGACTTTCCCTGCAAAGCGCTGGAGCCTTTCGGCATCGAAGCGCAGCA
TCCGGACGAGTTCGTCGACAACCTCTTGGGGCTTGATCCGGCAGCAGTGGTTGCAGCGGCCCAAAAACAGCGTCGGCAGC
TGAAAATGCCTGCCATGGACGTCGAACCTTTTCTTGATCTACTGCAACGACAAGGCCTGGTTAAGTCGGTCCAGGCACTC
AGCGGCTATCACGCGATCCTTTGA
ATGTGGCTTGCCCTCTCGGGGCGATTTCGGGCGCGATGGACCCAGGAGATTCACTGCGAATGGAAACGCAATCTGCTGAA
GAACCGCCCTGACCTGACGATGGAACAGCTGGATCGTACGTCCGCCCTGATGGATCAAGCGATACCCGACGCCTGTTTCC
ATGACTATGAAGACCTGATTGCCGGACTCAGCTTGCCCGACTGCGATGATCGTCATGTTCTGGCAGCGGCCATCCGTTGC
GGCGCAAGCGTGATAGTGACGTTCAACCAGAAAGACTTTCCCTGCAAAGCGCTGGAGCCTTTCGGCATCGAAGCGCAGCA
TCCGGACGAGTTCGTCGACAACCTCTTGGGGCTTGATCCGGCAGCAGTGGTTGCAGCGGCCCAAAAACAGCGTCGGCAGC
TGAAAATGCCTGCCATGGACGTCGAACCTTTTCTTGATCTACTGCAACGACAAGGCCTGGTTAAGTCGGTCCAGGCACTC
AGCGGCTATCACGCGATCCTTTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8362.31 Da Isoelectric Point: 4.7405
>AT232825 WP_006788213.1 NZ_CP091127:10502-10720 [Serratia marcescens]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT232825 NZ_CP128260:c2067295-2066828 [Pseudomonas fluorescens]
ATGTCGACCGTTATTCATCCCTCCATCAGCCTCCCCGTAGAACGCGAAGTAAAAGCCGCCATTGAAGGGCAGCGTGCCCT
GGCGGCTTACCTCGCGACCCATTTCGAGACTCAGCACATCCAGATTTTCGACGAGCAGAATGAAGCTCATCGCGTCGAAC
TGCCCACCTCGGCCCTGCGCTTGCTGATCGACATTCTGGCTGAGCTGGCAGCTGGCAACGCGGTTCAGGTCGTACCGGTG
CATGCCGAATTGACGACCCAGGAAGCCGCCGACCTGCTCAACGTCTCACGTCCACACCTCATAAAACTGCTGGAAAGCGG
CGAGTTGTCCTACCACAAAACAGGCAAGCACCGACGTGTACGTTTTGCCGATCTGATGGACTATAAGACCCGGCGCACCA
CCGCCAGCGAGCAGGCAATGGCGCTTCTCGCAGAGCAAGCTCAGGCGTTAAGGACAGGATACGAGTGA
ATGTCGACCGTTATTCATCCCTCCATCAGCCTCCCCGTAGAACGCGAAGTAAAAGCCGCCATTGAAGGGCAGCGTGCCCT
GGCGGCTTACCTCGCGACCCATTTCGAGACTCAGCACATCCAGATTTTCGACGAGCAGAATGAAGCTCATCGCGTCGAAC
TGCCCACCTCGGCCCTGCGCTTGCTGATCGACATTCTGGCTGAGCTGGCAGCTGGCAACGCGGTTCAGGTCGTACCGGTG
CATGCCGAATTGACGACCCAGGAAGCCGCCGACCTGCTCAACGTCTCACGTCCACACCTCATAAAACTGCTGGAAAGCGG
CGAGTTGTCCTACCACAAAACAGGCAAGCACCGACGTGTACGTTTTGCCGATCTGATGGACTATAAGACCCGGCGCACCA
CCGCCAGCGAGCAGGCAATGGCGCTTCTCGCAGAGCAAGCTCAGGCGTTAAGGACAGGATACGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2A5MCG5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1D8K3R4 |