Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 95177..96800 Replicon plasmid unnamed1
Accession NZ_CP091126
Organism Serratia marcescens strain CM2015_854

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag L2D47_RS27155 Protein ID WP_000088044.1
Coordinates 95475..96800 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag L2D47_RS27150 Protein ID WP_012477377.1
Coordinates 95177..95470 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2D47_RS27130 (L2D47_27125) 90961..91617 + 657 WP_000462754.1 trypsin-like serine protease -
L2D47_RS27135 (L2D47_27130) 91795..92763 - 969 WP_077254959.1 IS5-like element IS903B family transposase -
L2D47_RS27140 (L2D47_27135) 92886..93383 + 498 WP_000340139.1 membrane protein -
L2D47_RS27145 (L2D47_27140) 93388..94776 + 1389 WP_012695445.1 hypothetical protein -
L2D47_RS27150 (L2D47_27145) 95177..95470 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
L2D47_RS27155 (L2D47_27150) 95475..96800 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
L2D47_RS27160 (L2D47_27155) 96861..97067 + 207 WP_000134171.1 hypothetical protein -
L2D47_RS27165 (L2D47_27160) 97168..97578 + 411 WP_000985909.1 hypothetical protein -
L2D47_RS27170 (L2D47_27165) 97591..98115 + 525 WP_001805097.1 hypothetical protein -
L2D47_RS27175 (L2D47_27170) 98297..99301 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
L2D47_RS27180 (L2D47_27175) 99392..99826 - 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(D) / sul1 / qacE / ere(A) / aac(6')-IIc / aph(3')-Ia / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / aadA2 / blaSHV-12 - 1..295330 295330
- flank IS/Tn - - 91795..92763 968
- flank IS/Tn - - 98297..99301 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T232822 WP_000088044.1 NZ_CP091126:95475-96800 [Serratia marcescens]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T232822 NZ_CP128260:519184-519366 [Pseudomonas fluorescens]
ATGCGTAGTCGGGAAATGATCAGGATGATCGAGGAGGGTGGCTGGTATCTGGTCGCCGTCAAAGGCAGTCATCACCAATA
CAAGCATCCGGGCAAGCCAGGGAGGGTAACGATCAAGCATCCGGATTCGGATCTTCCCAAGGGTACGATCAACAGCATCT
TGAAACAGGCGGGTTTGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT232822 WP_012477377.1 NZ_CP091126:95177-95470 [Serratia marcescens]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT232822 NZ_CP128260:519426-519827 [Pseudomonas fluorescens]
ATGAAATTCCCGGTCGTTCTGCACAAGGATGCCGACTCAGACTACGGAGTGATTATCCCGGATGTACCGGGGTGCTTCTC
CGCAGGCACTACGGTAGCCGAAGCATTTGACAACACTCTGGAGGCATTGGCCTTGCACTATGAGGGATTGGTCGCTGATG
GCGCGCCGTTACCTCGGATCCAGGACATTGATGCCCATCTCGAGAATCCGGATTACGCCGGCGGTATCTGGGGTGTGGTC
GAGTTTGATATCACCCCCTATTTCGGCAAGTCGGTGCGCTTCAACGCTACGTTGCCGGAGCAGTTACTGGAGCGTATCGA
TCAGACGGTTCGGCGTGACCAGCGCTACAGTACCCGCTCCGGATTTCTGGCCGCTGCTGCCTTGCGCGAGTTGTCGGCAT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References