Detailed information of TA system TA232808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1287920..1288515
• Replicon: chromosome
• Accession: NZ_CP091125
• Organism: Serratia marcescens strain CM2015_854

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: L2D47_RS06380
• Protein ID: WP_071605322.1
• Coordinates: 1287920..1288174 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L2D47_RS06385
• Protein ID: WP_223294898.1
• Coordinates: 1288171..1288515 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D47_RS06345 (L2D47_06345)	1282929..1283231	+	303	WP_055312643.1	hypothetical protein	-
L2D47_RS06350 (L2D47_06350)	1283295..1283588	+	294	WP_085819519.1	DUF2732 family protein	-
L2D47_RS06355 (L2D47_06355)	1283588..1283812	+	225	WP_055312639.1	TraR/DksA C4-type zinc finger protein	-
L2D47_RS06360 (L2D47_06360)	1283935..1284216	+	282	WP_055312638.1	DUF5405 family protein	-
L2D47_RS06365 (L2D47_06365)	1284213..1286429	+	2217	WP_055312637.1	replication endonuclease	-
L2D47_RS06370 (L2D47_06370)	1286510..1286692	+	183	WP_085819361.1	hypothetical protein	-
L2D47_RS06375 (L2D47_06375)	1286913..1287398	+	486	WP_055312635.1	GNAT family N-acetyltransferase	-
L2D47_RS06380 (L2D47_06380)	1287920..1288174	+	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
L2D47_RS06385 (L2D47_06385)	1288171..1288515	+	345	WP_223294898.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
L2D47_RS06390 (L2D47_06390)	1288560..1289594	-	1035	WP_055312634.1	phage portal protein	-
L2D47_RS06395 (L2D47_06395)	1289594..1291366	-	1773	WP_055312633.1	terminase ATPase subunit family protein	-
L2D47_RS06400 (L2D47_06400)	1291509..1292324	+	816	WP_055312632.1	GPO family capsid scaffolding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1274885..1331527	56642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (56-101)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T232808 WP_071605322.1 NZ_CP091125:1287920-1288174 [Serratia marcescens]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T232808 NZ_CP128255:1996936-1997190 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12531.10 Da
• Isoelectric Point: 4.5789

>AT232808 WP_223294898.1 NZ_CP091125:1288171-1288515 [Serratia marcescens]
MMNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLS
PERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 345 bp

>AT232808 NZ_CP128255:1996688-1996939 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGTGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1