Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 595148..595770 Replicon chromosome
Accession NZ_CP091125
Organism Serratia marcescens strain CM2015_854

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag L2D47_RS02865 Protein ID WP_004940313.1
Coordinates 595148..595351 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag L2D47_RS02870 Protein ID WP_004940312.1
Coordinates 595402..595770 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2D47_RS02840 (L2D47_02840) 590956..591402 + 447 WP_047728676.1 type II secretion system protein GspM -
L2D47_RS02845 (L2D47_02845) 591439..592254 + 816 WP_055313416.1 A24 family peptidase -
L2D47_RS02850 (L2D47_02850) 592251..592613 - 363 WP_055313419.1 type II secretion system pilot lipoprotein GspS -
L2D47_RS02855 (L2D47_02855) 592866..594293 + 1428 WP_055313421.1 N-acetylglucosamine-binding protein GbpA -
L2D47_RS02860 (L2D47_02860) 594346..594864 + 519 WP_047728667.1 cytochrome b/b6 domain-containing protein -
L2D47_RS02865 (L2D47_02865) 595148..595351 - 204 WP_004940313.1 HHA domain-containing protein Toxin
L2D47_RS02870 (L2D47_02870) 595402..595770 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
L2D47_RS02875 (L2D47_02875) 595929..596282 - 354 WP_038874974.1 hypothetical protein -
L2D47_RS02880 (L2D47_02880) 596692..597405 + 714 WP_055312214.1 ABC transporter ATP-binding protein -
L2D47_RS02885 (L2D47_02885) 597402..598259 + 858 WP_038874980.1 metal ABC transporter permease -
L2D47_RS02890 (L2D47_02890) 598285..599163 + 879 WP_055312213.1 zinc ABC transporter substrate-binding protein -
L2D47_RS02895 (L2D47_02895) 599270..599410 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
L2D47_RS02900 (L2D47_02900) 599423..599677 - 255 WP_038874985.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T232806 WP_004940313.1 NZ_CP091125:c595351-595148 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T232806 NZ_CP128255:1154658-1154993 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT232806 WP_004940312.1 NZ_CP091125:c595770-595402 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT232806 NZ_CP128255:1154410-1154658 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGATCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References