Detailed information of TA system TA232803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 14279..14880
• Replicon: chromosome
• Accession: NZ_CP091125
• Organism: Serratia marcescens strain CM2015_854

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L2D47_RS00070
• Protein ID: WP_033636540.1
• Coordinates: 14279..14659 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0G3SPC0
• Locus tag: L2D47_RS00075
• Protein ID: WP_038875999.1
• Coordinates: 14659..14880 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D47_RS00045 (L2D47_00045)	9532..10812	+	1281	WP_055315561.1	DUF3748 domain-containing protein	-
L2D47_RS00050 (L2D47_00050)	10843..11190	-	348	WP_016929593.1	YceK/YidQ family lipoprotein	-
L2D47_RS00055 (L2D47_00055)	11500..11913	+	414	WP_004933919.1	small heat shock chaperone IbpA	-
L2D47_RS00060 (L2D47_00060)	12014..12442	+	429	WP_004933922.1	small heat shock chaperone IbpB	-
L2D47_RS00065 (L2D47_00065)	12610..14268	+	1659	WP_047729572.1	putative transporter	-
L2D47_RS00070 (L2D47_00070)	14279..14659	-	381	WP_033636540.1	type II toxin-antitoxin system VapC family toxin	Toxin
L2D47_RS00075 (L2D47_00075)	14659..14880	-	222	WP_038875999.1	ribbon-helix-helix domain-containing protein	Antitoxin
L2D47_RS00080 (L2D47_00080)	14955..16205	-	1251	WP_004933935.1	valine--pyruvate transaminase	-
L2D47_RS00085 (L2D47_00085)	16341..18404	-	2064	WP_055312216.1	alpha-amylase	-
L2D47_RS00090 (L2D47_00090)	18600..18752	+	153	WP_168166338.1	hypothetical protein	-
L2D47_RS00095 (L2D47_00095)	18754..19731	-	978	WP_016929588.1	glyoxylate/hydroxypyruvate reductase GhrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-102)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14185.53 Da
• Isoelectric Point: 7.3170

>T232803 WP_033636540.1 NZ_CP091125:c14659-14279 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T232803 NZ_CP128255:c621841-621377 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8563.50 Da
• Isoelectric Point: 4.4791

>AT232803 WP_038875999.1 NZ_CP091125:c14880-14659 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLAENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Nucleotide

• Length: 222 bp

>AT232803 NZ_CP128255:c622176-621841 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0G3SPC0