Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1014180..1014802 Replicon chromosome
Accession NZ_CP091123
Organism Serratia marcescens strain CM2017_569

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag L2D48_RS04765 Protein ID WP_004940313.1
Coordinates 1014180..1014383 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag L2D48_RS04770 Protein ID WP_004940312.1
Coordinates 1014434..1014802 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2D48_RS04745 (L2D48_04750) 1009249..1010139 + 891 WP_240296234.1 hypothetical protein -
L2D48_RS04750 (L2D48_04755) 1010669..1011340 + 672 WP_038876207.1 hypothetical protein -
L2D48_RS04755 (L2D48_04760) 1011337..1012449 + 1113 WP_017145378.1 ImmA/IrrE family metallo-endopeptidase -
L2D48_RS04760 (L2D48_04765) 1012620..1013787 - 1168 Protein_907 IS3 family transposase -
L2D48_RS04765 (L2D48_04770) 1014180..1014383 - 204 WP_004940313.1 HHA domain-containing protein Toxin
L2D48_RS04770 (L2D48_04775) 1014434..1014802 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
L2D48_RS04775 (L2D48_04780) 1014961..1015314 - 354 WP_038874974.1 hypothetical protein -
L2D48_RS04780 (L2D48_04785) 1015724..1016437 + 714 WP_038874977.1 ABC transporter ATP-binding protein -
L2D48_RS04785 (L2D48_04790) 1016434..1017291 + 858 WP_038874980.1 metal ABC transporter permease -
L2D48_RS04790 (L2D48_04795) 1017317..1018195 + 879 WP_038874983.1 zinc ABC transporter substrate-binding protein -
L2D48_RS04795 (L2D48_04800) 1018302..1018442 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
L2D48_RS04800 (L2D48_04805) 1018455..1018709 - 255 WP_038874985.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T232788 WP_004940313.1 NZ_CP091123:c1014383-1014180 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T232788 NZ_CP128233:1299459-1299716 [Paralcaligenes sp. CC-YST667]
ATGCGTGTCGTCAACTTCTCCGAGGCAAGAAACAGTCTGAAGCTCGTGATTGATCAAGTCATCAACGATGCCGATTACAC
GGTCATTGCACGTCGCGATGCCCCTGATGCGGTCGTGATGTCACTGGACACGTTCAATAGCCTTATGGAAACTGTCCATC
TGCTGAACTCACCAGCCAACGCGGCACATCTGGCACAATCCATCGCGCAATACCGTGAAGGTAACGTCATCACACAGGCG
CTTGACGATGCCGACTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT232788 WP_004940312.1 NZ_CP091123:c1014802-1014434 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT232788 NZ_CP128233:1299706-1299978 [Paralcaligenes sp. CC-YST667]
ATGCCGACTAGACCCGAGCCAAAACTGCTCTGGACGATTGCTGCCTGGCAAGATTACGAATACTGGCAAGGGCAAGACAA
GAAAACACTTAAACGCATCAATACCCTGATCAAAGACTGCATGCGCCATCCCTTTGAGGGTTTAGGCAAGCCTGAACCCC
TCAAAGAAAATTTGTCGGGTTTCTGGTCACGAAGAATAGATGACACACACCGTCTGGTTTACTGCGTCGACGAACAGGGG
CTTGTCATCATTGCATGCCGGTATCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References