Detailed information of TA system TA232781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4379177..4379772
• Replicon: chromosome
• Accession: NZ_CP091122
• Organism: Serratia marcescens strain CM2012_028

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: L2D51_RS20920
• Protein ID: WP_071605322.1
• Coordinates: 4379518..4379772 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L2D51_RS20915
• Protein ID: WP_240293734.1
• Coordinates: 4379177..4379521 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D51_RS20900 (L2D51_20900)	4374864..4376636	+	1773	WP_240293731.1	terminase ATPase subunit family protein	-
L2D51_RS20905 (L2D51_20905)	4376636..4377670	+	1035	WP_240293732.1	phage portal protein	-
L2D51_RS20910 (L2D51_20910)	4377701..4379011	-	1311	WP_240293733.1	DUF3644 domain-containing protein	-
L2D51_RS20915 (L2D51_20915)	4379177..4379521	-	345	WP_240293734.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
L2D51_RS20920 (L2D51_20920)	4379518..4379772	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
L2D51_RS20925 (L2D51_20925)	4380233..4381081	+	849	WP_240293735.1	hypothetical protein	-
L2D51_RS20930 (L2D51_20930)	4381148..4381315	-	168	WP_240293736.1	hypothetical protein	-
L2D51_RS20935 (L2D51_20935)	4381396..4383618	-	2223	WP_275891538.1	replication endonuclease	-
L2D51_RS20940 (L2D51_20940)	4383609..4384616	-	1008	WP_240293738.1	phosphoadenosine phosphosulfate reductase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4344389..4389051	44662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (56-101)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T232781 WP_071605322.1 NZ_CP091122:c4379772-4379518 [Serratia marcescens]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T232781 NZ_CP128219:c223385-223080 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12536.11 Da
• Isoelectric Point: 4.4537

>AT232781 WP_240293734.1 NZ_CP091122:c4379521-4379177 [Serratia marcescens]
MMNNTLKIDGHTAIINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECQKDGIEPYKSYSGKVTTRLS
PERHQALALAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 345 bp

>AT232781 NZ_CP128219:c223605-223387 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1