Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1076543..1077164 Replicon chromosome
Accession NZ_CP091122
Organism Serratia marcescens strain CM2012_028

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag L2D51_RS05110 Protein ID WP_004940313.1
Coordinates 1076543..1076746 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag L2D51_RS05115 Protein ID WP_004940312.1
Coordinates 1076796..1077164 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2D51_RS05080 (L2D51_05080) 1072244..1072582 + 339 WP_049299676.1 P-II family nitrogen regulator -
L2D51_RS05085 (L2D51_05085) 1072618..1073904 + 1287 WP_240293909.1 ammonium transporter AmtB -
L2D51_RS05090 (L2D51_05090) 1073995..1074858 - 864 WP_016928847.1 acyl-CoA thioesterase II -
L2D51_RS05095 (L2D51_05095) 1075089..1075580 + 492 WP_016928846.1 YbaY family lipoprotein -
L2D51_RS05100 (L2D51_05100) 1075645..1075959 - 315 WP_004940317.1 MGMT family protein -
L2D51_RS05110 (L2D51_05110) 1076543..1076746 - 204 WP_004940313.1 HHA domain-containing protein Toxin
L2D51_RS05115 (L2D51_05115) 1076796..1077164 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
L2D51_RS05120 (L2D51_05120) 1077323..1077676 - 354 WP_016928845.1 hypothetical protein -
L2D51_RS05125 (L2D51_05125) 1078081..1078794 + 714 WP_049299731.1 ABC transporter ATP-binding protein -
L2D51_RS05130 (L2D51_05130) 1078791..1079648 + 858 WP_143778568.1 metal ABC transporter permease -
L2D51_RS05135 (L2D51_05135) 1079674..1080552 + 879 WP_016928843.1 metal ABC transporter substrate-binding protein -
L2D51_RS05140 (L2D51_05140) 1080660..1080800 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
L2D51_RS05145 (L2D51_05145) 1080813..1081067 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T232767 WP_004940313.1 NZ_CP091122:c1076746-1076543 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T232767 NZ_CP128218:c2530587-2530252 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT232767 WP_004940312.1 NZ_CP091122:c1077164-1076796 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT232767 NZ_CP128218:c2530835-2530587 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References