Detailed information of TA system TA232753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1598678..1599225
• Replicon: chromosome
• Accession: NZ_CP091121
• Organism: Serratia ureilytica strain CM2016_324

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: L2D49_RS07635
• Protein ID: WP_089191399.1
• Coordinates: 1598917..1599225 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0G3SUU9
• Locus tag: L2D49_RS07630
• Protein ID: WP_033633717.1
• Coordinates: 1598678..1598914 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D49_RS07615 (L2D49_07615)	1595164..1596699	+	1536	WP_042784031.1	glutathione ABC transporter substrate-binding protein GsiB	-
L2D49_RS07620 (L2D49_07620)	1596752..1597672	+	921	WP_025302141.1	glutathione ABC transporter permease GsiC	-
L2D49_RS07625 (L2D49_07625)	1597682..1598590	+	909	WP_240290341.1	glutathione ABC transporter permease GsiD	-
L2D49_RS07630 (L2D49_07630)	1598678..1598914	+	237	WP_033633717.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
L2D49_RS07635 (L2D49_07635)	1598917..1599225	+	309	WP_089191399.1	CcdB family protein	Toxin
L2D49_RS07640 (L2D49_07640)	1599262..1600104	-	843	WP_015377214.1	S-formylglutathione hydrolase	-
L2D49_RS07645 (L2D49_07645)	1600119..1601243	-	1125	WP_016928454.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
L2D49_RS07650 (L2D49_07650)	1601274..1602194	-	921	WP_164107264.1	LysR family transcriptional regulator	-
L2D49_RS07655 (L2D49_07655)	1602300..1603457	+	1158	WP_042784036.1	YbfB/YjiJ family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11486.29 Da
• Isoelectric Point: 5.0196

>T232753 WP_089191399.1 NZ_CP091121:1598917-1599225 [Serratia ureilytica]
MQFTVYDNTYPASAYPYLLDVQSDLIDVLSTRLVIPLYALDNVRVKISARLCPEIEINGEKFLVMTHEMAAVRISQIGKA
VGNVNEHRNQIKAAIDFLIDGF

Nucleotide

• Length: 309 bp

>T232753 NZ_CP128218:c102916-102698 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8994.07 Da
• Isoelectric Point: 5.0807

>AT232753 WP_033633717.1 NZ_CP091121:1598678-1598914 [Serratia ureilytica]
MANNNVTKKTVNVTIDRDLFQRAKSLGVNVSSVLTDALSVRVRDIEIQQWREQNRAALEELNRITEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT232753 NZ_CP128218:c103316-102942 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0G3SUU9