Detailed information of TA system TA232752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1107893..1108515
• Replicon: chromosome
• Accession: NZ_CP091121
• Organism: Serratia ureilytica strain CM2016_324

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A8B4G7F9
• Locus tag: L2D49_RS05215
• Protein ID: WP_004940313.1
• Coordinates: 1107893..1108096 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A2X2G5J9
• Locus tag: L2D49_RS05220
• Protein ID: WP_004940312.1
• Coordinates: 1108147..1108515 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D49_RS05190 (L2D49_05190)	1103705..1104169	+	465	WP_033651100.1	type II secretion system protein GspM	-
L2D49_RS24580	1104296..1104463	+	168	Protein_992	prepilin peptidase	-
L2D49_RS24585	1104494..1105000	+	507	WP_275891534.1	A24 family peptidase	-
L2D49_RS05200 (L2D49_05200)	1104997..1105359	-	363	WP_147838717.1	type II secretion system pilot lipoprotein GspS	-
L2D49_RS05205 (L2D49_05205)	1105613..1107040	+	1428	WP_042783800.1	N-acetylglucosamine-binding protein GbpA	-
L2D49_RS05210 (L2D49_05210)	1107093..1107611	+	519	WP_033651103.1	cytochrome b/b6 domain-containing protein	-
L2D49_RS05215 (L2D49_05215)	1107893..1108096	-	204	WP_004940313.1	HHA domain-containing protein	Toxin
L2D49_RS05220 (L2D49_05220)	1108147..1108515	-	369	WP_004940312.1	Hha toxicity modulator TomB	Antitoxin
L2D49_RS05225 (L2D49_05225)	1108675..1109028	-	354	WP_047025751.1	hypothetical protein	-
L2D49_RS05230 (L2D49_05230)	1109436..1110149	+	714	WP_069100193.1	ABC transporter ATP-binding protein	-
L2D49_RS05235 (L2D49_05235)	1110146..1111003	+	858	WP_240290270.1	metal ABC transporter permease	-
L2D49_RS05240 (L2D49_05240)	1111029..1111907	+	879	WP_042783804.1	zinc ABC transporter substrate-binding protein	-
L2D49_RS05245 (L2D49_05245)	1112013..1112153	-	141	WP_004940304.1	type B 50S ribosomal protein L36	-
L2D49_RS05250 (L2D49_05250)	1112166..1112420	-	255	WP_004940303.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8107.37 Da
• Isoelectric Point: 6.9756

>T232752 WP_004940313.1 NZ_CP091121:c1108096-1107893 [Serratia ureilytica]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Nucleotide

• Length: 204 bp

>T232752 NZ_CP128214:1823157-1823426 [Agrobacterium fabrum]
GTGATCTGGACAATTGAATATCACACGCTTGTGCAGAAAGAGATGCGCAAAATAAATCCGGAAGTGCGCCGGCGCATCCG
CAGTTTTCTCCATGAAAGGCTGGCCGCACTGGACGACCCGCGCCAGATCGGCGCTACACTGCAAGGTTCCGAACTCGGAA
ATTTCTGGCGTTACCGGGTGGGCGATTACCGCATCATCTGCGATATACAGGACCAAAAGCTGGTCGTTCTGGTGGTCGAA
ATCGGCCATCGCCGTGAAATTTACCGTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14261.95 Da
• Isoelectric Point: 4.4314

>AT232752 WP_004940312.1 NZ_CP091121:c1108515-1108147 [Serratia ureilytica]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Nucleotide

• Length: 369 bp

>AT232752 NZ_CP128214:1822946-1823173 [Agrobacterium fabrum]
ATGAGCAAACAGACGGCCATTCGCCTGCCGGACGAGACCTATGAGCGGCTGAAGGCGCTGTCTGAGCGGACCGGCCGCAC
ATCCGCCTATTATATCCGCGAGGCGATCGAGAAGCATATCGAGGATATGGAAGACCTCTATCTTGCCGAAGAGGCGACGC
GGCGCATTCAGCGCGGAGAATCGAAGATTATAAGCGCCGAGGAGTTCTGGCGTGATCTGGACAATTGA

Structures

Toxin

• AlphaFold DB: A0A8B4G7F9

Antitoxin

• AlphaFold DB: A0A2X2G5J9