Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 14142..14743 Replicon chromosome
Accession NZ_CP091121
Organism Serratia ureilytica strain CM2016_324

Toxin (Protein)


Gene name vapC Uniprot ID A0A8B4GSJ7
Locus tag L2D49_RS00065 Protein ID WP_025304766.1
Coordinates 14142..14522 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A8B4GSF3
Locus tag L2D49_RS00070 Protein ID WP_025304767.1
Coordinates 14522..14743 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2D49_RS00040 (L2D49_00040) 9389..10669 + 1281 WP_033645140.1 DUF3748 domain-containing protein -
L2D49_RS00045 (L2D49_00045) 10700..11047 - 348 WP_016929593.1 YceK/YidQ family lipoprotein -
L2D49_RS00050 (L2D49_00050) 11357..11770 + 414 WP_004933919.1 small heat shock chaperone IbpA -
L2D49_RS00055 (L2D49_00055) 11877..12305 + 429 WP_004933922.1 small heat shock chaperone IbpB -
L2D49_RS00060 (L2D49_00060) 12473..14131 + 1659 WP_033652317.1 putative transporter -
L2D49_RS00065 (L2D49_00065) 14142..14522 - 381 WP_025304766.1 type II toxin-antitoxin system VapC family toxin Toxin
L2D49_RS00070 (L2D49_00070) 14522..14743 - 222 WP_025304767.1 ribbon-helix-helix domain-containing protein Antitoxin
L2D49_RS00075 (L2D49_00075) 14817..16067 - 1251 WP_047025992.1 valine--pyruvate transaminase -
L2D49_RS00080 (L2D49_00080) 17081..17860 + 780 WP_033645137.1 2OG-Fe dioxygenase family protein -
L2D49_RS00085 (L2D49_00085) 17940..19079 + 1140 WP_046687995.1 PLP-dependent aspartate aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-100)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-40)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14241.66 Da        Isoelectric Point: 7.3173

>T232750 WP_025304766.1 NZ_CP091121:c14522-14142 [Serratia ureilytica]
MVAQRALFDTNILIDYLNGIPQARDVLVEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRMLISRNPKDFGTDNGVLMPYRL

Download         Length: 381 bp

>T232750 NZ_CP128214:c1564452-1564093 [Agrobacterium fabrum]
ATGGTCCGCAACCAGATCCCCAAGCGCGGCGACGTTTATCTGGTTGATCTAAACCCTGTCGTAGGCAGCGAAATCAAGGA
CGAACATCGCTGTGTCGTCATCACGCCCAGAGAAATTAACGCGGTCGGACTCTGTCTCGTCGTCCCGGTGACCACCGGCG
GCATGTTTACGCGCAAGGCAGGGCTTGCCGTAAATATATCCGGCCACAAGACAACGGGCGTCGCTTTGTGCAATCAGGTG
AGAAGCATGGATATCGTCGCCCGGGTTGCCCAGAAGAAAGCGAAATATATCGAAACCCTCGATGATGCGACGATCGATGA
AATCGCCGGGCGCGTCATCAGCATGATCGATCCAGCTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8607.55 Da        Isoelectric Point: 4.4915

>AT232750 WP_025304767.1 NZ_CP091121:c14743-14522 [Serratia ureilytica]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVEYENRQREEWE

Download         Length: 222 bp

>AT232750 NZ_CP128214:c1564718-1564452 [Agrobacterium fabrum]
ATGACCGTGACCACGAAAATAAGACGCCAGGGCGGCGCGGCGGTGATGACGATCCCGCCCGCTCTTTTGAAGATGCTGGG
GCTGGAAATCGGTGAGCAACTGACGCTCGAGGTCGATAATGGCGCTCTGGTGGCAAGCCCGGTTCGACTGGAAAAGAAAC
GCTTCACGCTGGCCGAATTACTGGATGGTGCAGAGGAAGTGGCAGCTCTAAACGCCAGGGAGCGGGCGTGGGACACAGCG
CCTCCTGTCGGCAAAGAAGCGTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4GSJ7

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GSF3

References