Detailed information of TA system TA023275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 4290579..4291089
• Replicon: chromosome
• Accession: NC_011662
• Organism: Thauera sp. MZ1T

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C4KD68
• Locus tag: TMZ1T_RS18875
• Protein ID: WP_012586207.1
• Coordinates: 4290579..4290833 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: N6ZZ64
• Locus tag: TMZ1T_RS18880
• Protein ID: WP_004361138.1
• Coordinates: 4290823..4291089 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMZ1T_RS20735	4285597..4286022	-	426	WP_012586200.1	Txe/YoeB family addiction module toxin	-
TMZ1T_RS18830	4286006..4286287	-	282	WP_012586201.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
TMZ1T_RS18835	4286485..4286736	+	252	WP_012586202.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TMZ1T_RS20740	4286736..4287164	+	429	WP_012586203.1	type II toxin-antitoxin system VapC family toxin	-
TMZ1T_RS18845	4287195..4287623	-	429	WP_012586204.1	putative toxin-antitoxin system toxin component, PIN family	-
TMZ1T_RS18850	4287620..4287844	-	225	WP_012586205.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
TMZ1T_RS18860	4288144..4288416	+	273	Protein_3773	PIN domain-containing protein	-
TMZ1T_RS18865	4288511..4290196	-	1686	WP_012586206.1	IS1634 family transposase	-
TMZ1T_RS18870	4290305..4290508	+	204	WP_187283014.1	hypothetical protein	-
TMZ1T_RS18875	4290579..4290833	-	255	WP_012586207.1	Txe/YoeB family addiction module toxin	Toxin
TMZ1T_RS18880	4290823..4291089	-	267	WP_004361138.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
TMZ1T_RS18885	4291210..4291365	+	156	WP_144312187.1	hypothetical protein	-
TMZ1T_RS20745	4291367..4291659	+	293	Protein_3779	type II toxin-antitoxin system RelE/ParE family toxin	-
TMZ1T_RS18890	4291911..4292312	+	402	WP_012586208.1	pilin	-
TMZ1T_RS18895	4292407..4294353	+	1947	WP_012586209.1	hypothetical protein	-
TMZ1T_RS18900	4294350..4295207	+	858	WP_012586210.1	glycosyltransferase family 2 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4287620..4304144	16524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10230.63 Da
• Isoelectric Point: 8.0931

>T23275 WP_012586207.1 NC_011662:c4290833-4290579 [Thauera sp. MZ1T]
MQPRFSDHAWEDYLYWQETDRKQLKRINALIEDIRRHPFAGIGKPEPLKHQLAGFWSRRIDDRHRLVYAVESEVLLIAQC
RDHY

Nucleotide

• Length: 255 bp

>T23275 NC_011662:c4290833-4290579 [Thauera sp. MZ1T]
ATGCAGCCTAGGTTTTCGGATCACGCCTGGGAGGACTATCTCTACTGGCAAGAGACCGACCGCAAGCAGCTAAAGCGCAT
CAATGCGCTGATCGAGGACATCCGCCGCCATCCCTTTGCCGGCATCGGCAAGCCCGAGCCGCTCAAGCACCAGCTGGCGG
GGTTCTGGTCGCGACGCATCGATGACCGGCACCGGCTGGTGTATGCGGTCGAGAGTGAGGTGCTGCTGATCGCGCAGTGC
CGCGACCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9767.10 Da
• Isoelectric Point: 5.1052

>AT23275 WP_004361138.1 NC_011662:c4291089-4290823 [Thauera sp. MZ1T]
MEALTYSYTRTHLAQVMRSVNEDRAPVLVTTQRGKPVVIMSLDDYNALEETAYLLRSPKGAKRLMESVEQLRAGGGEPRA
LIETDDAA

Nucleotide

• Length: 267 bp

>AT23275 NC_011662:c4291089-4290823 [Thauera sp. MZ1T]
ATGGAAGCCCTGACCTACAGCTACACCCGCACGCACCTTGCGCAGGTGATGCGCAGCGTCAATGAAGACCGCGCGCCGGT
GCTGGTGACGACCCAGCGCGGCAAGCCGGTGGTGATCATGTCGCTGGACGACTACAACGCACTCGAAGAGACGGCCTATC
TGCTGCGCTCGCCGAAGGGTGCGAAGCGCCTGATGGAGTCGGTGGAGCAGTTGCGCGCAGGCGGTGGCGAGCCGCGTGCC
CTGATCGAGACGGACGATGCAGCCTAG

Structures

Toxin

• AlphaFold DB: C4KD68

Antitoxin

• AlphaFold DB: N6ZZ64