Detailed information of TA system TA232736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1563106..1563653
• Replicon: chromosome
• Accession: NZ_CP091120
• Organism: Serratia marcescens strain CM2019_254

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A380ADA6
• Locus tag: L2D50_RS07495
• Protein ID: WP_025302144.1
• Coordinates: 1563345..1563653 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: L2D50_RS07490
• Protein ID: WP_025302143.1
• Coordinates: 1563106..1563342 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D50_RS07475 (L2D50_07475)	1559592..1561127	+	1536	WP_025302140.1	glutathione ABC transporter substrate-binding protein GsiB	-
L2D50_RS07480 (L2D50_07480)	1561180..1562100	+	921	WP_025302141.1	glutathione ABC transporter permease GsiC	-
L2D50_RS07485 (L2D50_07485)	1562110..1563018	+	909	WP_169540348.1	glutathione ABC transporter permease GsiD	-
L2D50_RS07490 (L2D50_07490)	1563106..1563342	+	237	WP_025302143.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
L2D50_RS07495 (L2D50_07495)	1563345..1563653	+	309	WP_025302144.1	CcdB family protein	Toxin
L2D50_RS07500 (L2D50_07500)	1563693..1564535	-	843	WP_240275394.1	S-formylglutathione hydrolase	-
L2D50_RS07505 (L2D50_07505)	1564550..1565674	-	1125	WP_043142415.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
L2D50_RS07510 (L2D50_07510)	1565705..1566625	-	921	WP_049208871.1	LysR family transcriptional regulator	-
L2D50_RS07515 (L2D50_07515)	1566731..1567888	+	1158	WP_060447539.1	YbfB/YjiJ family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11504.32 Da
• Isoelectric Point: 5.0196

>T232736 WP_025302144.1 NZ_CP091120:1563345-1563653 [Serratia marcescens]
MQFTVYDNTYPASAYPYLLDVQSDLIDVLSTRLMIPLYALDNVRVKISARLCPEIEVNGEKFLVMTHEMAAVRISQIGKA
VGNVNEHRNQIKAAIDFLIDGF

Nucleotide

• Length: 309 bp

>T232736 NZ_CP128192:123197-123679 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8967.04 Da
• Isoelectric Point: 5.0807

>AT232736 WP_025302143.1 NZ_CP091120:1563106-1563342 [Serratia marcescens]
MANSNVTKKTVNVTIDRDLFQRAKSLGVNVSSVLTDALSVRVRDIEIQQWREQNRAALEELNRITEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT232736 NZ_CP128192:122928-123206 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A380ADA6