Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1085848..1086470 Replicon chromosome
Accession NZ_CP091120
Organism Serratia marcescens strain CM2019_254

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag L2D50_RS05060 Protein ID WP_004940313.1
Coordinates 1085848..1086051 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag L2D50_RS05065 Protein ID WP_004940312.1
Coordinates 1086102..1086470 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2D50_RS05030 (L2D50_05030) 1081547..1081885 + 339 WP_004940327.1 P-II family nitrogen regulator -
L2D50_RS05035 (L2D50_05035) 1081922..1083208 + 1287 WP_038628958.1 ammonium transporter AmtB -
L2D50_RS05040 (L2D50_05040) 1083299..1084162 - 864 WP_164099566.1 acyl-CoA thioesterase II -
L2D50_RS05045 (L2D50_05045) 1084394..1084885 + 492 WP_025301777.1 YbaY family lipoprotein -
L2D50_RS05050 (L2D50_05050) 1084950..1085264 - 315 WP_025301778.1 MGMT family protein -
L2D50_RS05060 (L2D50_05060) 1085848..1086051 - 204 WP_004940313.1 HHA domain-containing protein Toxin
L2D50_RS05065 (L2D50_05065) 1086102..1086470 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
L2D50_RS05070 (L2D50_05070) 1086629..1086982 - 354 WP_197790691.1 hypothetical protein -
L2D50_RS05075 (L2D50_05075) 1087390..1088100 + 711 WP_197790690.1 ABC transporter ATP-binding protein -
L2D50_RS05080 (L2D50_05080) 1088097..1088954 + 858 WP_043135664.1 metal ABC transporter permease -
L2D50_RS05085 (L2D50_05085) 1088980..1089858 + 879 WP_197790689.1 zinc ABC transporter substrate-binding protein -
L2D50_RS05090 (L2D50_05090) 1089965..1090105 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
L2D50_RS05095 (L2D50_05095) 1090118..1090372 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T232734 WP_004940313.1 NZ_CP091120:c1086051-1085848 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T232734 NZ_CP128192:46288-46599 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT232734 WP_004940312.1 NZ_CP091120:c1086470-1086102 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT232734 NZ_CP128192:46596-47015 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References