Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 14158..14759 Replicon chromosome
Accession NZ_CP091120
Organism Serratia marcescens strain CM2019_254

Toxin (Protein)


Gene name vapC Uniprot ID A0A8B4GSJ7
Locus tag L2D50_RS00070 Protein ID WP_025304766.1
Coordinates 14158..14538 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A8B4GSF3
Locus tag L2D50_RS00075 Protein ID WP_025304767.1
Coordinates 14538..14759 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2D50_RS00045 (L2D50_00045) 9405..10685 + 1281 WP_127146953.1 DUF3748 domain-containing protein -
L2D50_RS00050 (L2D50_00050) 10716..11063 - 348 WP_016929593.1 YceK/YidQ family lipoprotein -
L2D50_RS00055 (L2D50_00055) 11373..11786 + 414 WP_004933919.1 small heat shock chaperone IbpA -
L2D50_RS00060 (L2D50_00060) 11893..12321 + 429 WP_004933922.1 small heat shock chaperone IbpB -
L2D50_RS00065 (L2D50_00065) 12489..14147 + 1659 WP_127146954.1 putative transporter -
L2D50_RS00070 (L2D50_00070) 14158..14538 - 381 WP_025304766.1 type II toxin-antitoxin system VapC family toxin Toxin
L2D50_RS00075 (L2D50_00075) 14538..14759 - 222 WP_025304767.1 ribbon-helix-helix domain-containing protein Antitoxin
L2D50_RS00080 (L2D50_00080) 14834..16084 - 1251 WP_049204660.1 valine--pyruvate transaminase -
L2D50_RS00085 (L2D50_00085) 16220..18283 - 2064 WP_127146955.1 alpha-amylase -
L2D50_RS00090 (L2D50_00090) 18480..18632 + 153 WP_162837931.1 hypothetical protein -
L2D50_RS00095 (L2D50_00095) 18634..19611 - 978 WP_049204664.1 glyoxylate/hydroxypyruvate reductase GhrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-100)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-40)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14241.66 Da        Isoelectric Point: 7.3173

>T232733 WP_025304766.1 NZ_CP091120:c14538-14158 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQARDVLVEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRMLISRNPKDFGTDNGVLMPYRL

Download         Length: 381 bp

>T232733 NZ_CP128191:c5363033-5362650 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8607.55 Da        Isoelectric Point: 4.4915

>AT232733 WP_025304767.1 NZ_CP091120:c14759-14538 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVEYENRQREEWE

Download         Length: 222 bp

>AT232733 NZ_CP128191:c5363275-5363033 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4GSJ7

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GSF3

References