Detailed information of TA system TA232731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 93674..94782
• Replicon: plasmid pP47-180
• Accession: NZ_CP091103
• Organism: Enterococcus faecium strain P47

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: L1881_RS13450
• Protein ID: WP_000233000.1
• Coordinates: 93913..94782 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: L1881_RS13445
• Protein ID: WP_000205227.1
• Coordinates: 93674..93898 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1881_RS13425 (L1881_13425)	90413..90742	+	330	Protein_97	DNA topoisomerase	-
L1881_RS13430 (L1881_13430)	91053..91550	+	498	WP_002327635.1	DNA recombinase	-
L1881_RS13435 (L1881_13435)	91551..91967	+	417	WP_000323438.1	recombinase	-
L1881_RS13440 (L1881_13440)	91969..93531	+	1563	WP_000136908.1	recombinase family protein	-
L1881_RS13445 (L1881_13445)	93674..93898	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
L1881_RS13450 (L1881_13450)	93913..94782	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
L1881_RS13455 (L1881_13455)	94763..95155	+	393	WP_235692370.1	GNAT family N-acetyltransferase	-
L1881_RS13460 (L1881_13460)	95156..96595	+	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
L1881_RS13465 (L1881_13465)	96729..97355	+	627	WP_010785624.1	class I SAM-dependent methyltransferase	-
L1881_RS13470 (L1881_13470)	97388..98296	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
L1881_RS13475 (L1881_13475)	98293..98568	+	276	Protein_107	hypothetical protein	-
L1881_RS13480 (L1881_13480)	98636..99274	+	639	WP_235692371.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T232731 WP_000233000.1 NZ_CP091103:93913-94782 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T232731 NZ_CP128191:c4882699-4882415 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT232731 WP_000205227.1 NZ_CP091103:93674-93898 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT232731 NZ_CP128191:c4882931-4882689 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: R3JIN1