Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 52105..53242 Replicon plasmid pP47-61
Accession NZ_CP091102
Organism Enterococcus faecium strain P47

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag L1881_RS12885 Protein ID WP_001284311.1
Coordinates 52105..52968 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag L1881_RS12890 Protein ID WP_000301765.1
Coordinates 52970..53242 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1881_RS12860 (L1881_12860) 47367..47684 - 318 WP_002300567.1 hypothetical protein -
L1881_RS12865 (L1881_12865) 48468..48551 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
L1881_RS12870 (L1881_12870) 48670..49413 + 744 Protein_56 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
L1881_RS12875 (L1881_12875) 49950..50369 + 420 WP_016922374.1 fosfomycin resistance bacillithiol transferase FosB3 -
L1881_RS12880 (L1881_12880) 50591..51931 + 1341 WP_010728931.1 ISL3 family transposase -
L1881_RS12885 (L1881_12885) 52105..52968 - 864 WP_001284311.1 zeta toxin family protein Toxin
L1881_RS12890 (L1881_12890) 52970..53242 - 273 WP_000301765.1 antitoxin Antitoxin
L1881_RS12895 (L1881_12895) 53259..53474 - 216 WP_001835296.1 peptide-binding protein -
L1881_RS12900 (L1881_12900) 53566..54462 - 897 WP_001835294.1 ParA family protein -
L1881_RS12905 (L1881_12905) 54551..56695 - 2145 WP_000108741.1 type IA DNA topoisomerase -
L1881_RS12910 (L1881_12910) 56695..57312 - 618 WP_001062589.1 recombinase family protein -
L1881_RS12915 (L1881_12915) 57326..57496 - 171 WP_000713594.1 hypothetical protein -
L1881_RS12920 (L1881_12920) 57853..58035 - 183 Protein_66 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(A) / optrA / fexA / erm(B) / fosB3 - 1..61338 61338


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T232730 WP_001284311.1 NZ_CP091102:c52968-52105 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T232730 NZ_CP128191:c4778578-4778045 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT232730 WP_000301765.1 NZ_CP091102:c53242-52970 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT232730 NZ_CP128191:c4778855-4778589 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References