Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2333103..2333674 Replicon chromosome
Accession NZ_CP091100
Organism Enterococcus faecium strain P47

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag L1881_RS11305 Protein ID WP_002302307.1
Coordinates 2333333..2333674 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag L1881_RS11300 Protein ID WP_002323011.1
Coordinates 2333103..2333333 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1881_RS11275 (L1881_11275) 2328592..2329920 + 1329 WP_010718318.1 FAD-dependent oxidoreductase -
L1881_RS11280 (L1881_11280) 2329942..2330568 + 627 WP_002286816.1 cysteine hydrolase -
L1881_RS11285 (L1881_11285) 2330751..2331332 + 582 WP_043902871.1 TetR/AcrR family transcriptional regulator -
L1881_RS11290 (L1881_11290) 2331697..2332272 + 576 WP_010718317.1 SOS response-associated peptidase family protein -
L1881_RS11295 (L1881_11295) 2332477..2332815 - 339 WP_002286804.1 hypothetical protein -
L1881_RS11300 (L1881_11300) 2333103..2333333 + 231 WP_002323011.1 hypothetical protein Antitoxin
L1881_RS11305 (L1881_11305) 2333333..2333674 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L1881_RS11310 (L1881_11310) 2334524..2334709 + 186 WP_002304207.1 hypothetical protein -
L1881_RS11320 (L1881_11320) 2336228..2336470 + 243 Protein_2176 LPXTG cell wall anchor domain-containing protein -
L1881_RS11325 (L1881_11325) 2336544..2337437 + 894 WP_002326335.1 class C sortase -
L1881_RS11330 (L1881_11330) 2337620..2338294 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T232729 WP_002302307.1 NZ_CP091100:2333333-2333674 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T232729 NZ_CP128191:4165478-4165696 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT232729 WP_002323011.1 NZ_CP091100:2333103-2333333 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT232729 NZ_CP128191:4165077-4165451 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References