Detailed information of TA system TA232727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2436918..2437549
• Replicon: chromosome
• Accession: NZ_CP091099
• Organism: Pantoea agglomerans strain Pa58

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7X5MR53
• Locus tag: LZ609_RS15170
• Protein ID: WP_010253953.1
• Coordinates: 2437370..2437549 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A8X8DTB6
• Locus tag: LZ609_RS15165
• Protein ID: WP_010253955.1
• Coordinates: 2436918..2437343 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ609_RS15145 (LZ609_15145)	2433622..2434131	-	510	WP_010253965.1	phenolic acid decarboxylase	-
LZ609_RS15150 (LZ609_15150)	2434237..2435136	+	900	WP_245115536.1	LysR family transcriptional regulator	-
LZ609_RS15155 (LZ609_15155)	2435581..2436510	-	930	WP_033770970.1	Rpn family recombination-promoting nuclease/putative transposase	-
LZ609_RS15160 (LZ609_15160)	2436747..2436836	-	90	Protein_2275	CRISPR-associated protein Cas1	-
LZ609_RS15165 (LZ609_15165)	2436918..2437343	-	426	WP_010253955.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LZ609_RS15170 (LZ609_15170)	2437370..2437549	-	180	WP_010253953.1	type II toxin-antitoxin system HicA family toxin	Toxin
LZ609_RS15175 (LZ609_15175)	2437732..2439156	-	1425	WP_245116859.1	dihydrolipoyl dehydrogenase	-
LZ609_RS15180 (LZ609_15180)	2439317..2441212	-	1896	WP_245115538.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6467.52 Da
• Isoelectric Point: 10.7891

>T232727 WP_010253953.1 NZ_CP091099:c2437549-2437370 [Pantoea agglomerans]
MDSSTLISEMKADGWVLTGINGSHHHFTHPTKPGLVTVPHPKKDLPIGTVKSIRKQARI

Nucleotide

• Length: 180 bp

>T232727 NZ_CP128191:2377705-2379027 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15422.60 Da
• Isoelectric Point: 4.7662

>AT232727 WP_010253955.1 NZ_CP091099:c2437343-2436918 [Pantoea agglomerans]
MFYPIAIEAGDHEHAYGVIVPDLPGCFSAGDTLDEAIKNAKEAITGHIELCVELGHEIPAVSTIETLATNPDYQGYIWAL
VDVDVIRLLGGSEKINVTLPRSLIDRIDRCVASHPEYKSRSGFLAQVALERITAPINRPQK

Nucleotide

• Length: 426 bp

>AT232727 NZ_CP128191:2377439-2377705 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7X5MR53