Detailed information of TA system TA232726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2182308..2182925
• Replicon: chromosome
• Accession: NZ_CP091099
• Organism: Pantoea agglomerans strain Pa58

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: E0LTU7
• Locus tag: LZ609_RS13960
• Protein ID: WP_003850458.1
• Coordinates: 2182710..2182925 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: E0LTU8
• Locus tag: LZ609_RS13955
• Protein ID: WP_003850455.1
• Coordinates: 2182308..2182685 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ609_RS13925 (LZ609_13925)	2178615..2178869	+	255	WP_010671219.1	type B 50S ribosomal protein L31	-
LZ609_RS13930 (LZ609_13930)	2178881..2179021	+	141	WP_010255896.1	type B 50S ribosomal protein L36	-
LZ609_RS13935 (LZ609_13935)	2179067..2179945	-	879	WP_245115424.1	metal ABC transporter substrate-binding protein	-
LZ609_RS13940 (LZ609_13940)	2179961..2180800	-	840	WP_010671217.1	metal ABC transporter permease	-
LZ609_RS13945 (LZ609_13945)	2180797..2181465	-	669	WP_033766689.1	ABC transporter ATP-binding protein	-
LZ609_RS13950 (LZ609_13950)	2181807..2182160	+	354	WP_122459125.1	hypothetical protein	-
LZ609_RS13955 (LZ609_13955)	2182308..2182685	+	378	WP_003850455.1	Hha toxicity modulator TomB	Antitoxin
LZ609_RS13960 (LZ609_13960)	2182710..2182925	+	216	WP_003850458.1	HHA domain-containing protein	Toxin
LZ609_RS13970 (LZ609_13970)	2183332..2183643	+	312	WP_031293095.1	MGMT family protein	-
LZ609_RS13975 (LZ609_13975)	2183683..2184240	-	558	WP_010255881.1	YbaY family lipoprotein	-
LZ609_RS13980 (LZ609_13980)	2184431..2185294	+	864	WP_010255880.1	acyl-CoA thioesterase II	-
LZ609_RS13985 (LZ609_13985)	2185345..2186631	-	1287	WP_010255877.1	ammonium transporter AmtB	-
LZ609_RS13990 (LZ609_13990)	2186666..2187004	-	339	WP_003850469.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (12-68)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 8510.90 Da
• Isoelectric Point: 9.4825

>T232726 WP_003850458.1 NZ_CP091099:2182710-2182925 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Nucleotide

• Length: 216 bp

>T232726 NZ_CP128191:933155-933562 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 14636.25 Da
• Isoelectric Point: 4.3976

>AT232726 WP_003850455.1 NZ_CP091099:2182308-2182685 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Nucleotide

• Length: 378 bp

>AT232726 NZ_CP128191:932908-933174 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A1I5AGC3

Antitoxin

• AlphaFold DB: A0A1I5AG55