Detailed information of TA system TA232725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1433108..1433692
• Replicon: chromosome
• Accession: NZ_CP091099
• Organism: Pantoea agglomerans strain Pa58

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LZ609_RS10310
• Protein ID: WP_111533731.1
• Coordinates: 1433357..1433692 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LZ609_RS10305
• Protein ID: WP_010245791.1
• Coordinates: 1433108..1433356 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ609_RS10285 (LZ609_10285)	1429343..1429954	-	612	WP_010245779.1	DUF2291 domain-containing protein	-
LZ609_RS10290 (LZ609_10290)	1430000..1430935	-	936	WP_010670004.1	D-ribose ABC transporter substrate-binding protein	-
LZ609_RS10295 (LZ609_10295)	1430991..1431788	-	798	WP_033771597.1	D-threitol dehydrogenase	-
LZ609_RS10300 (LZ609_10300)	1432141..1432953	+	813	WP_010245789.1	DeoR/GlpR family DNA-binding transcription regulator	-
LZ609_RS10305 (LZ609_10305)	1433108..1433356	+	249	WP_010245791.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LZ609_RS10310 (LZ609_10310)	1433357..1433692	+	336	WP_111533731.1	endoribonuclease MazF	Toxin
LZ609_RS10315 (LZ609_10315)	1434026..1434379	+	354	WP_031292988.1	DUF6404 family protein	-
LZ609_RS10320 (LZ609_10320)	1434989..1435828	-	840	WP_245116844.1	uroporphyrinogen-III C-methyltransferase	-
LZ609_RS10325 (LZ609_10325)	1435828..1438446	-	2619	WP_158131758.1	nitrate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-108)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12020.02 Da
• Isoelectric Point: 9.2246

>T232725 WP_111533731.1 NZ_CP091099:1433357-1433692 [Pantoea agglomerans]
MVSRFVPDAGDIIWVDFDPTKGSEQAGHRPAIVLSPFAYNNKTGMCLCVPCTTLSKGYPFEVPISAEKESVALSDQVKSI
AWKVRGATKKGVVSANELQQIRAKIKVIIGL

Nucleotide

• Length: 336 bp

>T232725 NZ_CP128191:795855-796340 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9271.61 Da
• Isoelectric Point: 4.1826

>AT232725 WP_010245791.1 NZ_CP091099:1433108-1433356 [Pantoea agglomerans]
MIHGNVKRWGNSPAIRIPATLMQALNLNIDDEIKIDLVEGKLIIEPVKQEVGYSLEELLEGVTEENLHDPVEWGEPVGKE
IW

Nucleotide

• Length: 249 bp

>AT232725 NZ_CP128191:795565-795858 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA