Detailed information of TA system TA232700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 254635..255231
• Replicon: chromosome
• Accession: NZ_CP091081
• Organism: Enterobacter roggenkampii strain GD21SC1505

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0F1DED9
• Locus tag: L1S38_RS01230
• Protein ID: WP_023293553.1
• Coordinates: 254929..255231 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: L1S38_RS01225
• Protein ID: WP_059360225.1
• Coordinates: 254635..254922 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1S38_RS01220 (L1S38_01220)	253007..254638	+	1632	WP_155020456.1	Na/Pi cotransporter family protein	-
L1S38_RS01225 (L1S38_01225)	254635..254922	-	288	WP_059360225.1	putative addiction module antidote protein	Antitoxin
L1S38_RS01230 (L1S38_01230)	254929..255231	-	303	WP_023293553.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L1S38_RS01235 (L1S38_01235)	255429..256301	+	873	WP_023293552.1	23S rRNA pseudouridine(2604) synthase RluF	-
L1S38_RS01240 (L1S38_01240)	256302..256574	-	273	WP_059360216.1	DUF3811 domain-containing protein	-
L1S38_RS01245 (L1S38_01245)	256625..257569	-	945	WP_023293551.1	ketopantoate/pantoate/pantothenate transporter PanS	-
L1S38_RS01250 (L1S38_01250)	257675..259249	-	1575	WP_250670272.1	RNA repair transcriptional activator RtcR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-93)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11459.24 Da
• Isoelectric Point: 10.1771

>T232700 WP_023293553.1 NZ_CP091081:c255231-254929 [Enterobacter roggenkampii]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDVKPVGEGISELRIHFGPGYRIYFKDQGNYIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T232700 NZ_CP128116:519325-519675 [Bacillus halotolerans]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAGATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10138.54 Da
• Isoelectric Point: 6.4596

>AT232700 WP_059360225.1 NZ_CP091081:c254922-254635 [Enterobacter roggenkampii]
MHKLTPYDPANALVDDEEIAVFIADALETGDSTYIAKALGVVARAKGISNISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGFGLTLKHAGD

Nucleotide

• Length: 288 bp

>AT232700 NZ_CP128116:519039-519320 [Bacillus halotolerans]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCCTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAACATTTCTTCCGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A0F1DED9