23270

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 91674..92199 Replicon plasmid pMAR2
Accession NC_011603
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag E2348C_RS25950 Protein ID WP_001159871.1
Coordinates 91674..91979 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q0H051
Locus tag E2348C_RS25955 Protein ID WP_000813637.1
Coordinates 91981..92199 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS28635 87223..88211 + 989 Protein_107 translesion error-prone DNA polymerase V subunit UmuC -
E2348C_RS25925 88216..88608 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
E2348C_RS25930 88613..89584 - 972 WP_001103698.1 hypothetical protein -
E2348C_RS25935 89813..90457 + 645 WP_000633911.1 AAA family ATPase -
E2348C_RS25940 90451..90726 + 276 WP_000239529.1 hypothetical protein -
E2348C_RS25945 90864..91673 - 810 WP_000016979.1 site-specific integrase -
E2348C_RS25950 91674..91979 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
E2348C_RS25955 91981..92199 - 219 WP_000813637.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E2348C_RS25960 92913..95534 + 2622 WP_000283686.1 hypothetical protein -
E2348C_RS25965 95733..95963 + 231 WP_001261274.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
E2348C_RS28900 96139..96327 + 189 WP_001386137.1 hypothetical protein -
E2348C_RS28840 96332..96549 - 218 Protein_118 transposase -
E2348C_RS25975 96550..97080 - 531 WP_000348885.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - bfpA / bfpG / bfpB / bfpC / bfpU / bfpD / bfpE / bfpF / bfpP / bfpH / bfpI / bfpJ / bfpK / bfpL / toxB / toxB 1..97978 97978


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T23270 WP_001159871.1 NC_011603:c91979-91674 [Escherichia coli O127:H6 str. E2348/69]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T23270 NC_011603:c91979-91674 [Escherichia coli O127:H6 str. E2348/69]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8428.45 Da        Isoelectric Point: 4.7405

>AT23270 WP_000813637.1 NC_011603:c92199-91981 [Escherichia coli O127:H6 str. E2348/69]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKVENQEGMVEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT23270 NC_011603:c92199-91981 [Escherichia coli O127:H6 str. E2348/69]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGTGGAAAATCAGGAAGGGATGGTTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB Q0H051

References