Detailed information of TA system TA232699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 45740..46401
• Replicon: chromosome
• Accession: NZ_CP091081
• Organism: Enterobacter roggenkampii strain GD21SC1505

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L1S38_RS00230
• Protein ID: WP_155020498.1
• Coordinates: 46003..46401 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A156KC23
• Locus tag: L1S38_RS00225
• Protein ID: WP_032675702.1
• Coordinates: 45740..46006 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1S38_RS00200 (L1S38_00200)	41793..43121	-	1329	WP_025910681.1	PTS sugar transporter subunit IIC	-
L1S38_RS00205 (L1S38_00205)	43133..43447	-	315	WP_155020497.1	PTS sugar transporter subunit IIB	-
L1S38_RS00210 (L1S38_00210)	43741..44691	+	951	WP_250670241.1	LacI family DNA-binding transcriptional regulator	-
L1S38_RS00215 (L1S38_00215)	44753..45049	-	297	WP_059365122.1	YicS family protein	-
L1S38_RS00220 (L1S38_00220)	45191..45610	+	420	WP_008500129.1	GNAT family N-acetyltransferase	-
L1S38_RS00225 (L1S38_00225)	45740..46006	+	267	WP_032675702.1	virulence protein	Antitoxin
L1S38_RS00230 (L1S38_00230)	46003..46401	+	399	WP_155020498.1	type II toxin-antitoxin system VapC family toxin	Toxin
L1S38_RS00235 (L1S38_00235)	46541..47341	+	801	WP_155020499.1	lipoprotein NlpA	-
L1S38_RS00240 (L1S38_00240)	47464..48243	+	780	WP_126348546.1	MBL fold metallo-hydrolase	-
L1S38_RS00245 (L1S38_00245)	48270..49223	+	954	WP_044597401.1	helix-turn-helix domain-containing protein	-
L1S38_RS00250 (L1S38_00250)	49408..50388	+	981	WP_032636617.1	IS5-like element IS5 family transposase	-
L1S38_RS00255 (L1S38_00255)	50480..51177	+	698	WP_227028264.1	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14826.07 Da
• Isoelectric Point: 6.8547

>T232699 WP_155020498.1 NZ_CP091081:46003-46401 [Enterobacter roggenkampii]
MRHMLDTNIVSHLFKRHPEVVSRMTCLAPGDVCISSITEAELLYGADKKKSSRLKETIREFLNTIKICDWDSDAAATYGE
LRATLEKKGKVMGDLDQLIAAHAISRGTTIVTNDRAFRMVQELAVEDWTTTL

Nucleotide

• Length: 399 bp

>T232699 NZ_CP128114:c1790163-1789852 [Bacillus safensis]
ATGACACCAATAGAAACAGCTAAGCATAGAGCAGAATTTGATAAAGTAAAAAATACAGTAATCAAAAAGTGGGAAGAAAA
TACTGGGCAAAAGTGGCCTGAGTATAATGAAAATGTTATTTCAGAAAAAACAGGTAAAATAATTAGGAAAAAAGGAGATA
AGTATGATGCTCATCACATAATTGAGAATACATTTGGAGGAGAGCATGAATGGTGGAATATGCATCCTGCAAAATTCCCA
AATGAGCATCAAGCTGGTATTCATGGGAAAGGTTCACCAGCAAACGAACTATTTAAAGGAGGTAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10009.34 Da
• Isoelectric Point: 4.6310

>AT232699 WP_032675702.1 NZ_CP091081:45740-46006 [Enterobacter roggenkampii]
MERTAKLFRKGQNQAVMLPAEFAFDTESVYIRRDEEGNVVLAAKPVLIKSWDNFLRMLAETDVPDTFLSKEERNQSVTTR
DPLDGACS

Nucleotide

• Length: 267 bp

>AT232699 NZ_CP128114:c1789852-1789412 [Bacillus safensis]
ATGGGATACGAATCTTTGAAGGCAAATCAAGAAAATAGTTTTTATCCAGTAACCGAAAATGAAATAAAAGAGGTTGAGAA
GGAACTTGATTTAAAATTTCCTAAAGAGTTAGTTAATTTTTATATGGAGGTTGGCTACGGGTTTATTAAAGGTTCAGAGT
TTAATAACAATCGTATTATGGATCCTTATTCCGTCAGAGATTTTCGATTAAGAGTTAATGATTTTGAGTTTTATCCTGAT
ATAGAAATCTATGATGAATTTGAAAATAACAAACTAATATTTTTTGAAGGTAGTGAATCAGCTTTAATGTCAATTGAATT
GAATGAAAAGAATAGCAGTCCGGTTTACTATTATGATATTCAAATTGCGACTTCTCTTGAAGAATTTTTAAGAAAAATAG
AAGAAAATGATAAATATTACTTGGAGTTACTAGTTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A156KC23