Detailed information of TA system TA232698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 14325..14872
• Replicon: plasmid pR1
• Accession: NZ_CP091080
• Organism: Xanthomonas arboricola pv. pruni strain R1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K9U01_RS22250
• Protein ID: WP_009873354.1
• Coordinates: 14546..14872 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K9U01_RS22245
• Protein ID: WP_043149934.1
• Coordinates: 14325..14549 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U01_RS22225 (K9U01_22225)	9917..10600	-	684	WP_009873358.1	protein mobD	-
K9U01_RS22230 (K9U01_22230)	10613..10969	-	357	WP_009873357.1	hypothetical protein	-
K9U01_RS22235 (K9U01_22235)	11285..11596	+	312	WP_009873356.1	ribbon-helix-helix protein, CopG family	-
K9U01_RS22240 (K9U01_22240)	11586..14258	+	2673	WP_047353284.1	DNA-primase RepB domain-containing protein	-
K9U01_RS22245 (K9U01_22245)	14325..14549	+	225	WP_043149934.1	antitoxin MazE family protein	Antitoxin
K9U01_RS22250 (K9U01_22250)	14546..14872	+	327	WP_009873354.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K9U01_RS22255 (K9U01_22255)	14869..15195	+	327	WP_009873353.1	hypothetical protein	-
K9U01_RS22260 (K9U01_22260)	15224..16102	+	879	WP_223571302.1	helicase RepA family protein	-
K9U01_RS22265 (K9U01_22265)	16083..17075	+	993	WP_009873351.1	replication protein C, IncQ-type	-
K9U01_RS22270 (K9U01_22270)	17272..18108	-	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
K9U01_RS22275 (K9U01_22275)	18108..18911	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
K9U01_RS22280 (K9U01_22280)	18977..19591	-	615	WP_000904906.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(C) / aph(6)-Id / aph(3'')-Ib	-	1..20174	20174
	-	flank	IS/Tn	aph(6)-Id / aph(3'')-Ib	-	17272..19591	2319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11487.49 Da
• Isoelectric Point: 9.2954

>T232698 WP_009873354.1 NZ_CP091080:14546-14872 [Xanthomonas arboricola pv. pruni]
MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTLNAAPLLRVTVQPSAENGLQKPSQVMVDKAMTVLRSK
VGPAFGRIDADALLEVERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T232698 NZ_CP128114:537681-538031 [Bacillus safensis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTGATGCAAAGCGCTACGGCTTTGAGCGAGATTCTGTCATTCTCTTGGAGCAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8468.56 Da
• Isoelectric Point: 5.0038

>AT232698 WP_043149934.1 NZ_CP091080:14325-14549 [Xanthomonas arboricola pv. pruni]
MATAHVNQRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCLLVAQADSADTAMQQFMDEALADVDGWTE

Nucleotide

• Length: 225 bp

>AT232698 NZ_CP128114:537395-537676 [Bacillus safensis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTACCGGAGGCTTTGATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA