Detailed information of TA system TA232694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 9252..9799
• Replicon: plasmid pT1
• Accession: NZ_CP091077
• Organism: Xanthomonas arboricola pv. pruni strain T1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K9U02_RS22255
• Protein ID: WP_009873354.1
• Coordinates: 9252..9578 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K9U02_RS22260
• Protein ID: WP_043149934.1
• Coordinates: 9575..9799 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U02_RS22220 (K9U02_22220)	4356..4411	+	56	Protein_6	hypothetical protein	-
K9U02_RS22225 (K9U02_22225)	4965..5192	+	228	Protein_7	helix-turn-helix domain-containing protein	-
K9U02_RS22230 (K9U02_22230)	5258..6061	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
K9U02_RS22235 (K9U02_22235)	6061..6897	+	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
K9U02_RS22240 (K9U02_22240)	7049..8041	-	993	WP_009873351.1	replication protein C, IncQ-type	-
K9U02_RS22245 (K9U02_22245)	8022..8900	-	879	WP_009873352.1	helicase RepA family protein	-
K9U02_RS22250 (K9U02_22250)	8929..9255	-	327	WP_009873353.1	hypothetical protein	-
K9U02_RS22255 (K9U02_22255)	9252..9578	-	327	WP_009873354.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K9U02_RS22260 (K9U02_22260)	9575..9799	-	225	WP_043149934.1	antitoxin MazE family protein	Antitoxin
K9U02_RS22265 (K9U02_22265)	9866..12538	-	2673	WP_047353284.1	DNA-primase RepB domain-containing protein	-
K9U02_RS22270 (K9U02_22270)	12528..12839	-	312	WP_009873356.1	ribbon-helix-helix protein, CopG family	-
K9U02_RS22275 (K9U02_22275)	13155..13511	+	357	WP_009873357.1	hypothetical protein	-
K9U02_RS22280 (K9U02_22280)	13524..14207	+	684	WP_009873358.1	protein mobD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(C) / aph(3'')-Ib / aph(6)-Id	-	1..15112	15112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11487.49 Da
• Isoelectric Point: 9.2954

>T232694 WP_009873354.1 NZ_CP091077:c9578-9252 [Xanthomonas arboricola pv. pruni]
MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTLNAAPLLRVTVQPSAENGLQKPSQVMVDKAMTVLRSK
VGPAFGRIDADALLEVERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T232694 NZ_CP128107:2363306-2364046 [Bacillus cereus]
TTGACGATCTCTAACATTCGAATCGGTTTATTTATTCTAGTAATCGCCTTTGTAGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACTTGGTATGGTTTGTTTATAATATCAGTCACAGTTTATT
TCATGATAAAAGGAGTAGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCTAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAGCAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAACTTAAACGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTGTTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTAGTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAACAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8468.56 Da
• Isoelectric Point: 5.0038

>AT232694 WP_043149934.1 NZ_CP091077:c9799-9575 [Xanthomonas arboricola pv. pruni]
MATAHVNQRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCLLVAQADSADTAMQQFMDEALADVDGWTE

Nucleotide

• Length: 225 bp

>AT232694 NZ_CP128107:2364159-2364284 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTTTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA