Detailed information of TA system TA232689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4429..5009
• Replicon: plasmid pWSCRKP-4
• Accession: NZ_CP091072
• Organism: Klebsiella pneumoniae strain WSCRKP

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: L0980_RS29360
• Protein ID: WP_071177730.1
• Coordinates: 4429..4743 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: L0980_RS29365
• Protein ID: WP_000093040.1
• Coordinates: 4731..5009 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0980_RS29335 (L0980_29335)	373..2337	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
L0980_RS29340 (L0980_29340)	2337..3068	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
L0980_RS29345 (L0980_29345)	3075..3605	+	531	WP_071177729.1	hypothetical protein	-
L0980_RS29350 (L0980_29350)	3632..3811	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
L0980_RS29355 (L0980_29355)	3837..4265	-	429	WP_001140599.1	hypothetical protein	-
L0980_RS29360 (L0980_29360)	4429..4743	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0980_RS29365 (L0980_29365)	4731..5009	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
L0980_RS29370 (L0980_29370)	5184..5549	-	366	WP_072354022.1	TonB family protein	-
L0980_RS29375 (L0980_29375)	5546..5917	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
L0980_RS29380 (L0980_29380)	6191..6436	-	246	WP_032440458.1	hypothetical protein	-
L0980_RS29385 (L0980_29385)	7081..8568	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..15931	15931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T232689 WP_071177730.1 NZ_CP091072:c4743-4429 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T232689 NZ_CP127881:4068301-4068645 [Serratia marcescens]
ATGTGGCAAGTGGTGACCGTCGAACGGTTTGACGACTGGTTTTTAGCGCTGAACAACGCCCAGCAAACAAGCATCCTGGC
GGCGATCTACAAATTGCAGACGTTCGGCCCGCAATTGGCGCGGCCGCATGCCGATACGCTGCATTTTTCCGACGCGGTCC
GGCAGTTGAAGGAGCTGCGCATTCAACATCGCGGGCGCCCTTTCAGGGTGTTTTTCGCCTTTGATCCACAGCGGCAAGCG
GTATTGCTGTGCGGCGGCGATAAAACCGGCGACAAACGCTTTTACCAGCGCATGTTGCCCATCGCCGCCATGGAGTTTTC
ACATTATCTGGCAACCCGGAGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT232689 WP_000093040.1 NZ_CP091072:c5009-4731 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT232689 NZ_CP127881:4068651-4068953 [Serratia marcescens]
ATGGCCAAACCTTTATCCCAACTGATCGATAAACTCGATCCCGCCGTCGTTGCCGCCGCCAGGCAAAAAGCGGACCAGGA
GATTTTCGAGCTGCGGCTGGCGATGCTGAGGGAAGAGTTGGCGGTTTCTCAGGTTGAATTGGCGAAGCGGCTCGGCATCA
GCCAACCCTCCGTCGCCAATTTGGAAAAGCGCGGCAGCGAAATCAAACTGTCATCGCTCAAACGCTATATCGAGGCGATG
GGCGGCACGCTGTCGCTGGATGTGCAATTGCCCAATGGCCAGCACCGCCGCATGACGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1