Detailed information of TA system TA232683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 145900..146570
• Replicon: plasmid pWSCRKP-1
• Accession: NZ_CP091069
• Organism: Klebsiella pneumoniae strain WSCRKP

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: L0980_RS27705
• Protein ID: WP_004213072.1
• Coordinates: 145900..146343 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: L0980_RS27710
• Protein ID: WP_004213073.1
• Coordinates: 146340..146570 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0980_RS27670 (L0980_27670)	141311..141586	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
L0980_RS27675 (L0980_27675)	141649..142140	+	492	WP_011251327.1	DM13 domain-containing protein	-
L0980_RS27680 (L0980_27680)	142189..143109	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
L0980_RS27685 (L0980_27685)	143200..143603	+	404	Protein_158	GAF domain-containing protein	-
L0980_RS27690 (L0980_27690)	144121..144756	-	636	Protein_159	mucoid phenotype regulator RmpA2	-
L0980_RS27695 (L0980_27695)	145173..145477	+	305	Protein_160	transposase	-
L0980_RS27700 (L0980_27700)	145500..145751	-	252	WP_186987481.1	hypothetical protein	-
L0980_RS27705 (L0980_27705)	145900..146343	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0980_RS27710 (L0980_27710)	146340..146570	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0980_RS27715 (L0980_27715)	147178..148311	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
L0980_RS27720 (L0980_27720)	148327..148620	+	294	WP_004213076.1	hypothetical protein	-
L0980_RS27725 (L0980_27725)	148610..148816	-	207	WP_004213077.1	hypothetical protein	-
L0980_RS27730 (L0980_27730)	149168..149458	+	291	WP_004213078.1	hypothetical protein	-
L0980_RS27735 (L0980_27735)	149448..150347	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..221776	221776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T232683 WP_004213072.1 NZ_CP091069:c146343-145900 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T232683 NZ_CP127881:c1681827-1681543 [Serratia marcescens]
ATGACTTATAAGCTTGAATTCGAAGAACATGCGCTGAAGGAATTTAAAAAACTGTCCCCGGTGATCCGGGAACAGTTCAA
AAAAAAGCTGGCATCGGTGCTGGTTAATCCCCACGTCCCCGCCAACAAACTCGCCGGTTTACCCGATTGCTACAAAATCA
AACTCAGAGCCTCAGGCTTTCGTTTGGTATATCGCGTCATGGAAACGGAGATCGTGGTTCTGGTCCTGTCTGTGGGCAAA
CGCGAACGTTCAGCGGCTTACACGGCTGCCAAAAAGCGCCTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT232683 WP_004213073.1 NZ_CP091069:c146570-146340 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT232683 NZ_CP127881:c1682068-1681817 [Serratia marcescens]
ATGACGGTTCAACCCATTCTTGCCGACTCGGCAGTCAGCATCAGCGATTTTAAGAAATCGCCCAACGCCGCCCTTAAAGA
GGCACAAGGGCAACCGGTCGCCGTCCTGACCAACGGTAAGATTTCGGGATATTACGTCTCTCCGGAAACCTGGGAGGCGA
TCGCGGAGTACCTGGAAGATATCGAACTCGCTGAAATCGTTCGTTCACGGATGAACGGCAAACGCATTAAGGTCAATTTG
GATGACTTATAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620