Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5381689..5382314 Replicon chromosome
Accession NZ_CP091068
Organism Klebsiella pneumoniae strain WSCRKP

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag L0980_RS26540 Protein ID WP_002882817.1
Coordinates 5381689..5382072 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L0980_RS26545 Protein ID WP_004150355.1
Coordinates 5382072..5382314 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0980_RS26525 (L0980_26525) 5379055..5379957 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L0980_RS26530 (L0980_26530) 5379954..5380589 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L0980_RS26535 (L0980_26535) 5380586..5381515 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L0980_RS26540 (L0980_26540) 5381689..5382072 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
L0980_RS26545 (L0980_26545) 5382072..5382314 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L0980_RS26550 (L0980_26550) 5382519..5383436 + 918 WP_004181612.1 alpha/beta hydrolase -
L0980_RS26555 (L0980_26555) 5383450..5384391 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L0980_RS26560 (L0980_26560) 5384436..5384873 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L0980_RS26565 (L0980_26565) 5384870..5385730 - 861 WP_004146232.1 virulence factor BrkB family protein -
L0980_RS26570 (L0980_26570) 5385724..5386323 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T232680 WP_002882817.1 NZ_CP091068:c5382072-5381689 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T232680 NZ_CP091068:c5382072-5381689 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT232680 WP_004150355.1 NZ_CP091068:c5382314-5382072 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT232680 NZ_CP091068:c5382314-5382072 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References