Detailed information of TA system TA023268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 29608..30143
• Replicon: plasmid pMAR2
• Accession: NC_011603
• Organism: Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: E2348C_RS25555
• Protein ID: WP_000222766.1
• Coordinates: 29608..29895 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: E2348C_RS25560
• Protein ID: WP_001132900.1
• Coordinates: 29892..30143 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2348C_RS28615	26597..27023	+	427	Protein_30	IS3 family transposase	-
E2348C_RS25535	27091..27527	-	437	Protein_31	ATP-binding protein	-
E2348C_RS25540	27540..28058	-	519	Protein_32	transposase	-
E2348C_RS25545	28145..29136	+	992	Protein_33	IS21 family transposase	-
E2348C_RS25550	29133..29402	+	270	Protein_34	transposase	-
E2348C_RS25555	29608..29895	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E2348C_RS25560	29892..30143	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E2348C_RS25565	30216..30407	-	192	WP_000841007.1	hypothetical protein	-
E2348C_RS25570	31108..31965	-	858	WP_000131012.1	incFII family plasmid replication initiator RepA	-
E2348C_RS25575	31958..32032	-	75	WP_001365705.1	RepA leader peptide Tap	-
E2348C_RS25580	32279..32527	-	249	WP_000083838.1	replication regulatory protein RepA	-
E2348C_RS26150	32811..32960	-	150	WP_001300273.1	type I toxin-antitoxin system Hok family toxin	-
E2348C_RS25585	33149..34522	+	1374	WP_000244358.1	IS4-like element ISEc13 family transposase	-
E2348C_RS25590	34555..35112	-	558	WP_000139319.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	bfpA / bfpG / bfpB / bfpC / bfpU / bfpD / bfpE / bfpF / bfpP / bfpH / bfpI / bfpJ / bfpK / bfpL / toxB / toxB	1..97978	97978
	-	inside	IScluster/Tn	-	toxB / toxB	15490..34522	19032

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T23268 WP_000222766.1 NC_011603:c29895-29608 [Escherichia coli O127:H6 str. E2348/69]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T23268 NC_011603:c29895-29608 [Escherichia coli O127:H6 str. E2348/69]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT23268 WP_001132900.1 NC_011603:c30143-29892 [Escherichia coli O127:H6 str. E2348/69]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT23268 NC_011603:c30143-29892 [Escherichia coli O127:H6 str. E2348/69]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2

Antitoxin

• AlphaFold DB: V0VIP2