Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4193321..4193940 Replicon chromosome
Accession NZ_CP091068
Organism Klebsiella pneumoniae strain WSCRKP

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag L0980_RS20850 Protein ID WP_002892050.1
Coordinates 4193722..4193940 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag L0980_RS20845 Protein ID WP_002892066.1
Coordinates 4193321..4193695 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0980_RS20835 (L0980_20835) 4188473..4189666 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L0980_RS20840 (L0980_20840) 4189689..4192835 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
L0980_RS20845 (L0980_20845) 4193321..4193695 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
L0980_RS20850 (L0980_20850) 4193722..4193940 + 219 WP_002892050.1 HHA domain-containing protein Toxin
L0980_RS20855 (L0980_20855) 4194099..4194665 + 567 WP_002892030.1 maltose O-acetyltransferase -
L0980_RS20860 (L0980_20860) 4194637..4194777 - 141 WP_004147370.1 hypothetical protein -
L0980_RS20865 (L0980_20865) 4194798..4195268 + 471 WP_002892026.1 YlaC family protein -
L0980_RS20870 (L0980_20870) 4195243..4196694 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
L0980_RS20875 (L0980_20875) 4196795..4197493 + 699 WP_002892021.1 GNAT family protein -
L0980_RS20880 (L0980_20880) 4197490..4197630 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
L0980_RS20885 (L0980_20885) 4197630..4197893 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T232676 WP_002892050.1 NZ_CP091068:4193722-4193940 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T232676 NZ_CP127873:4491748-4491966 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT232676 WP_002892066.1 NZ_CP091068:4193321-4193695 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT232676 NZ_CP127873:4491347-4491721 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTTGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGTTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References