Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4700724..4701221 Replicon chromosome
Accession NC_011601
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag E2348C_RS23980 Protein ID WP_001173343.1
Coordinates 4701048..4701221 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag E2348C_RS23975 Protein ID WP_000398619.1
Coordinates 4700724..4701020 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS23955 (E2348_C_4451) 4697454..4698173 - 720 WP_000611288.1 two-component system response regulator DcuR -
E2348C_RS23960 (E2348_C_4452) 4698170..4699801 - 1632 WP_001216494.1 sensor histidine kinase -
E2348C_RS23965 (E2348_C_4453) 4699982..4700212 + 231 WP_000371707.1 (4Fe-4S)-binding protein -
E2348C_RS23970 (E2348_C_4454) 4700224..4700496 + 273 WP_000405647.1 N-acetyltransferase -
E2348C_RS23975 (E2348_C_4455) 4700724..4701020 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
E2348C_RS23980 4701048..4701221 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
E2348C_RS23985 (E2348_C_4456) 4701340..4702857 - 1518 WP_000003801.1 lysine--tRNA ligase -
E2348C_RS23990 (E2348_C_4457) 4703094..4704551 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T23267 WP_001173343.1 NC_011601:4701048-4701221 [Escherichia coli O127:H6 str. E2348/69]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T23267 NC_011601:4701048-4701221 [Escherichia coli O127:H6 str. E2348/69]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT23267 WP_000398619.1 NC_011601:4700724-4701020 [Escherichia coli O127:H6 str. E2348/69]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT23267 NC_011601:4700724-4701020 [Escherichia coli O127:H6 str. E2348/69]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References