Detailed information of TA system TA232659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2746954..2747609
• Replicon: chromosome
• Accession: NZ_CP091067
• Organism: Pseudomonas sp. BC42

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D6BKI7
• Locus tag: L1O02_RS12800
• Protein ID: WP_060839909.1
• Coordinates: 2747430..2747609 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L1O02_RS12795
• Protein ID: WP_235760018.1
• Coordinates: 2746954..2747379 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O02_RS12770 (L1O02_12770)	2741992..2742564	-	573	WP_235760014.1	type 1 glutamine amidotransferase family protein	-
L1O02_RS12775 (L1O02_12775)	2742792..2743616	+	825	WP_235760015.1	hypothetical protein	-
L1O02_RS12780 (L1O02_12780)	2743753..2744535	+	783	WP_146189207.1	hypothetical protein	-
L1O02_RS12785 (L1O02_12785)	2744713..2746146	+	1434	WP_235760016.1	RtcB family protein	-
L1O02_RS12790 (L1O02_12790)	2746337..2746882	+	546	WP_235760017.1	hypothetical protein	-
L1O02_RS12795 (L1O02_12795)	2746954..2747379	-	426	WP_235760018.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
L1O02_RS12800 (L1O02_12800)	2747430..2747609	-	180	WP_060839909.1	type II toxin-antitoxin system HicA family toxin	Toxin
L1O02_RS12805 (L1O02_12805)	2747699..2747965	-	267	WP_060843956.1	hypothetical protein	-
L1O02_RS12810 (L1O02_12810)	2748398..2748787	+	390	WP_060843955.1	RidA family protein	-
L1O02_RS12815 (L1O02_12815)	2748907..2749671	+	765	WP_235760019.1	IclR family transcriptional regulator	-
L1O02_RS12820 (L1O02_12820)	2749719..2750936	+	1218	WP_235760020.1	amino acid deaminase	-
L1O02_RS12825 (L1O02_12825)	2751083..2752000	+	918	WP_235760021.1	sugar kinase	-
L1O02_RS12830 (L1O02_12830)	2752109..2752384	-	276	WP_011061856.1	peptidylprolyl isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6632.63 Da
• Isoelectric Point: 11.0565

>T232659 WP_060839909.1 NZ_CP091067:c2747609-2747430 [Pseudomonas sp. BC42]
MKFSEFRRWLKAQGVVFEAGKGSHFKVTAPNGNQTTFADHGSKEMPEPTRKAIIRQLGL

Nucleotide

• Length: 180 bp

>T232659 NZ_CP127869:1958797-1958985 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTAGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGTATCTTATAA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15626.99 Da
• Isoelectric Point: 5.1799

>AT232659 WP_235760018.1 NZ_CP091067:c2747379-2746954 [Pseudomonas sp. BC42]
MYDYALRFEQDRAPGLAVFCRDLPELNSFGDDPGHAINQAIDALETTLSLYVDQRRAIPPASAPLPGEQVIHLPMVTVAK
IALWNTMMERDMRKADLCRLLGIHQAQGDRLVDFLHSSKMEQLENALAALGKRLAVSVEAA

Nucleotide

• Length: 426 bp

>AT232659 NZ_CP127869:1958515-1958772 [Staphylococcus epidermidis]
ATGAAATTAAACAAAACAAATATATTCAACTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTGGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATTTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTATATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: A0A0D6BKI7