Detailed information of TA system TA232653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2090969..2091483
• Replicon: chromosome
• Accession: NZ_CP091066
• Organism: Staphylococcus aureus strain UMCG579

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: L1O91_RS10225
• Protein ID: WP_054190442.1
• Coordinates: 2090969..2091178 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: L1O91_RS10230
• Protein ID: WP_000138934.1
• Coordinates: 2091190..2091483 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O91_RS10205 (2086477)	2086477..2087835	-	1359	WP_054190439.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
L1O91_RS10210 (2087850)	2087850..2088920	-	1071	WP_054190440.1	D-alanine--D-alanine ligase	-
L1O91_RS10215 (2089238)	2089238..2090440	+	1203	WP_054190441.1	FtsW/RodA/SpoVE family cell cycle protein	-
L1O91_RS10220 (2090704)	2090704..2090841	-	138	WP_000828354.1	Lmo0850 family protein	-
L1O91_RS10225 (2090969)	2090969..2091178	-	210	WP_054190442.1	heavy metal-associated domain-containing protein	Toxin
L1O91_RS10230 (2091190)	2091190..2091483	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
L1O91_RS10235 (2091653)	2091653..2093137	+	1485	WP_054190443.1	cardiolipin synthase	-
L1O91_RS10240 (2093165)	2093165..2093812	+	648	WP_001187616.1	HD domain-containing protein	-
L1O91_RS10245 (2094392)	2094392..2095264	-	873	WP_000725800.1	membrane protein insertase YidC	-
L1O91_RS10250 (2095350)	2095350..2095991	-	642	WP_054190444.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7830.77 Da
• Isoelectric Point: 4.1008

>T232653 WP_054190442.1 NZ_CP091066:c2091178-2090969 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISSMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKILF

Nucleotide

• Length: 210 bp

>T232653 NZ_CP127855:c3773684-3773391 [Pseudoalteromonas sp. JC3]
ATGATTTATAAACTCGGTTTTAAAAAGAGTGCACTCAAAGAATGGAGTAAACTAGGCTCTACCCTGCAATCGCAGTTCAA
GAAAAAGTTAATTGAACGCCTCAAGGATCCCCATGTGCCAGCGTCGAAGCTATCTGGTGCTGACAACATGTACAAAATAA
AACTACGCCAATCAGGTTACCGCCTAGTCTACAAAGTTGAAGACGACATTATCGTTGTAACTATGCTAGCAGTGGGAAAG
CGTGAACGTAGTGATGTTTATCATAAGGCTATGAAGCGAATAAAAGATGATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT232653 WP_000138934.1 NZ_CP091066:c2091483-2091190 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT232653 NZ_CP127855:c3773922-3773674 [Pseudoalteromonas sp. JC3]
ATGACCACTAGAATATTAGCAGATGTTGCTGCGAGCATTACTGAACTAAAAGCAAACCCTATGAAAGTTGCAACAAGTGC
TGACGGAGAACCTGTCGCTGTGCTTAATCGAAATGAACCTGCTTTTTATTGTGTGCCTGCAGAAGCATACGAGATAATGA
TGGACAGGCTCGAGGATCTTGAGCTACTCGCTATTGCTAAAGAGCGTGAATCTGAAGAAAGTATCTCAATAAATATTAAT
GATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0D1GBN9