Detailed information of TA system TA232652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2071228..2071757
• Replicon: chromosome
• Accession: NZ_CP091066
• Organism: Staphylococcus aureus strain UMCG579

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L1O91_RS10135
• Protein ID: WP_000621175.1
• Coordinates: 2071228..2071590 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: L1O91_RS10140
• Protein ID: WP_000948331.1
• Coordinates: 2071587..2071757 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O91_RS10115 (2068206)	2068206..2068976	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
L1O91_RS10120 (2068951)	2068951..2069430	-	480	WP_054190427.1	anti-sigma B factor RsbW	-
L1O91_RS10125 (2069432)	2069432..2069758	-	327	WP_001052491.1	anti-sigma factor antagonist	-
L1O91_RS10130 (2069877)	2069877..2070878	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
L1O91_RS10135 (2071228)	2071228..2071590	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L1O91_RS10140 (2071587)	2071587..2071757	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
L1O91_RS10145 (2071842)	2071842..2072990	-	1149	WP_054190429.1	alanine racemase	-
L1O91_RS10150 (2073056)	2073056..2073415	-	360	WP_000581197.1	holo-ACP synthase	-
L1O91_RS10155 (2073419)	2073419..2073910	-	492	WP_054190430.1	PH domain-containing protein	-
L1O91_RS10160 (2073897)	2073897..2075480	-	1584	WP_054190431.1	PH domain-containing protein	-
L1O91_RS10165 (2075473)	2075473..2075952	-	480	WP_054190432.1	hypothetical protein	-
L1O91_RS10170 (2076161)	2076161..2076721	-	561	WP_054190433.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T232652 WP_000621175.1 NZ_CP091066:c2071590-2071228 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T232652 NZ_CP127855:2149849-2150136 [Pseudoalteromonas sp. JC3]
ATGTCAGTAAATTTTAAAGATGATTGGCTTGAAGCATTTTATGAGGAAGATAAGCGTCATCGCTTGATTCCTAAAACTAT
AGAGAATGCGCTGTATCGAAAGCTTGAAATCCTAGATGCAGCAAAAGCTGAATCAGATCTAAGAGTTCCACCGGGTAATC
GGTTTGAACATCTTGAAGGCAATCTGAAAGGTTGGTGTTCGATACGGGTGAATAAACAGTATCGTTTGATTTTTAAGTGG
GTTGACGGTGTAGCAGAAGACACCTATTTAGACCCTCATAAGTACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT232652 WP_000948331.1 NZ_CP091066:c2071757-2071587 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT232652 NZ_CP127855:2150147-2150464 [Pseudoalteromonas sp. JC3]
ATGCGCAGAACTAAACGTCGTCCAGTTGGTGTAGGAGAAATGCTCAAAGTTGAATTCTTGGAGCCAATGGAGATTACATC
AAAAACATTAGCAGAAGCTATGGGTGTACATAGGAACACCATCAGTAACTTAATTAATGGTGGTACTCTGACTGCTCCAC
TTGCAATCAAGCTAGCGGCTGCACTTGGGAATACACCTGAGTTTTGGCTTAACATTCAACATGCTGTGGACTTGTGGGAT
ACACGAAATCGTTATCAAGATGAAGCCAAGCAAGTAAAACCTTTGTTTTCAACGCAAGATATCAAACTACGTACATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1