Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3869482..3870113 Replicon chromosome
Accession NC_011601
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag E2348C_RS19985 Protein ID WP_001260301.1
Coordinates 3869838..3870113 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E2348C_RS19980 Protein ID WP_000593555.1
Coordinates 3869482..3869841 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS19955 (E2348_C_3718) 3865614..3866558 + 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
E2348C_RS19960 (E2348_C_3719) 3866555..3867388 + 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
E2348C_RS19965 (E2348_C_3720) 3867388..3868152 + 765 WP_001136232.1 nickel import ATP-binding protein NikD -
E2348C_RS19970 (E2348_C_3721) 3868149..3868955 + 807 WP_000173679.1 nickel import ATP-binding protein NikE -
E2348C_RS19975 (E2348_C_3722) 3868961..3869362 + 402 WP_001190063.1 nickel-responsive transcriptional regulator NikR -
E2348C_RS19980 (E2348_C_3723) 3869482..3869841 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E2348C_RS19985 (E2348_C_3724) 3869838..3870113 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
E2348C_RS19990 (E2348_C_3725) 3870186..3871310 - 1125 WP_001314210.1 ABC transporter permease -
E2348C_RS19995 (E2348_C_3726) 3871310..3874045 - 2736 WP_000149096.1 ribosome-associated ATPase/putative transporter RbbA -
E2348C_RS20000 (E2348_C_3727) 3874042..3875109 - 1068 WP_000361455.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T23264 WP_001260301.1 NC_011601:c3870113-3869838 [Escherichia coli O127:H6 str. E2348/69]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T23264 NC_011601:c3870113-3869838 [Escherichia coli O127:H6 str. E2348/69]
ATGAGAACCCAGGTACAGGCTTTGCGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT23264 WP_000593555.1 NC_011601:c3869841-3869482 [Escherichia coli O127:H6 str. E2348/69]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT23264 NC_011601:c3869841-3869482 [Escherichia coli O127:H6 str. E2348/69]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References