Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3269063..3269717 Replicon chromosome
Accession NC_011601
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag E2348C_RS16895 Protein ID WP_000244781.1
Coordinates 3269063..3269470 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag E2348C_RS16900 Protein ID WP_000354046.1
Coordinates 3269451..3269717 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS16875 (E2348_C_3145) 3265020..3266753 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
E2348C_RS16880 (E2348_C_3146) 3266759..3267469 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
E2348C_RS16885 (E2348_C_3147) 3267494..3268390 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
E2348C_RS16890 (E2348_C_3148) 3268502..3269023 + 522 WP_001055874.1 flavodoxin FldB -
E2348C_RS16895 (E2348_C_3149) 3269063..3269470 - 408 WP_000244781.1 protein YgfX Toxin
E2348C_RS16900 (E2348_C_3150) 3269451..3269717 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
E2348C_RS16905 (E2348_C_3151) 3269960..3270940 + 981 WP_000886076.1 tRNA-modifying protein YgfZ -
E2348C_RS16910 (E2348_C_3152) 3271017..3271676 - 660 WP_000250274.1 hemolysin III family protein -
E2348C_RS16915 (E2348_C_3153) 3271840..3272151 - 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
E2348C_RS16920 (E2348_C_3154) 3272196..3273629 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T23263 WP_000244781.1 NC_011601:c3269470-3269063 [Escherichia coli O127:H6 str. E2348/69]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T23263 NC_011601:c3269470-3269063 [Escherichia coli O127:H6 str. E2348/69]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT23263 WP_000354046.1 NC_011601:c3269717-3269451 [Escherichia coli O127:H6 str. E2348/69]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT23263 NC_011601:c3269717-3269451 [Escherichia coli O127:H6 str. E2348/69]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References