Detailed information of TA system TA232629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2190931..2191569
• Replicon: chromosome
• Accession: NZ_CP091065
• Organism: Escherichia coli strain DOC14

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: L1123_RS10655
• Protein ID: WP_000813794.1
• Coordinates: 2191393..2191569 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L1123_RS10650
• Protein ID: WP_001270286.1
• Coordinates: 2190931..2191347 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1123_RS10630 (2186083)	2186083..2187024	-	942	WP_001251304.1	ABC transporter permease	-
L1123_RS10635 (2187025)	2187025..2188038	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
L1123_RS10640 (2188056)	2188056..2189201	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
L1123_RS10645 (2189446)	2189446..2190852	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
L1123_RS10650 (2190931)	2190931..2191347	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
L1123_RS10655 (2191393)	2191393..2191569	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
L1123_RS10660 (2191791)	2191791..2192021	+	231	WP_000494244.1	YncJ family protein	-
L1123_RS10665 (2192113)	2192113..2194074	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
L1123_RS10670 (2194147)	2194147..2194683	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
L1123_RS10675 (2194736)	2194736..2195950	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T232629 WP_000813794.1 NZ_CP091065:c2191569-2191393 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T232629 NZ_CP127848:294469-294699 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT232629 WP_001270286.1 NZ_CP091065:c2191347-2190931 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT232629 NZ_CP127848:294696-295055 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• PDB: 6HPB