Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3142708..3143291 Replicon chromosome
Accession NC_011601
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag E2348C_RS16355 Protein ID WP_000254750.1
Coordinates 3142708..3143043 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag E2348C_RS16360 Protein ID WP_000581937.1
Coordinates 3143043..3143291 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS16340 (E2348_C_3046) 3138594..3139892 - 1299 WP_000036723.1 phosphopyruvate hydratase -
E2348C_RS16345 (E2348_C_3047) 3139980..3141617 - 1638 WP_000210870.1 CTP synthase (glutamine hydrolyzing) -
E2348C_RS16350 (E2348_C_3048) 3141845..3142636 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
E2348C_RS16355 (E2348_C_3049) 3142708..3143043 - 336 WP_000254750.1 endoribonuclease MazF Toxin
E2348C_RS16360 (E2348_C_3050) 3143043..3143291 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
E2348C_RS16365 (E2348_C_3051) 3143369..3145603 - 2235 WP_000226815.1 GTP pyrophosphokinase -
E2348C_RS16370 (E2348_C_3052) 3145651..3146952 - 1302 WP_000046793.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T23262 WP_000254750.1 NC_011601:c3143043-3142708 [Escherichia coli O127:H6 str. E2348/69]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T23262 NC_011601:c3143043-3142708 [Escherichia coli O127:H6 str. E2348/69]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCTCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT23262 WP_000581937.1 NC_011601:c3143291-3143043 [Escherichia coli O127:H6 str. E2348/69]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT23262 NC_011601:c3143291-3143043 [Escherichia coli O127:H6 str. E2348/69]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References