Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1623688..1624519 Replicon chromosome
Accession NZ_CP091065
Organism Escherichia coli strain DOC14

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag L1123_RS07765 Protein ID WP_000854814.1
Coordinates 1623688..1624062 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag L1123_RS07770 Protein ID WP_001285584.1
Coordinates 1624151..1624519 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1123_RS07725 (1619084) 1619084..1620250 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
L1123_RS07730 (1620369) 1620369..1620842 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
L1123_RS07735 (1621040) 1621040..1622098 + 1059 WP_001200891.1 FUSC family protein -
L1123_RS07740 (1622270) 1622270..1622599 + 330 WP_000450409.1 DUF496 family protein -
L1123_RS07745 (1622700) 1622700..1622834 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
L1123_RS07750 (1622954) 1622954..1623082 + 129 Protein_1516 transposase domain-containing protein -
L1123_RS07755 (1623371) 1623371..1623451 - 81 Protein_1517 hypothetical protein -
L1123_RS07760 (1623497) 1623497..1623691 - 195 WP_000988600.1 DUF5983 family protein -
L1123_RS07765 (1623688) 1623688..1624062 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
L1123_RS07770 (1624151) 1624151..1624519 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
L1123_RS07775 (1624593) 1624593..1624814 - 222 WP_000692323.1 DUF987 domain-containing protein -
L1123_RS07780 (1624877) 1624877..1625323 - 447 WP_000187523.1 RadC family protein -
L1123_RS07785 (1625320) 1625320..1626852 - 1533 WP_001350525.1 protein YeeR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T232618 WP_000854814.1 NZ_CP091065:c1624062-1623688 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T232618 NZ_CP127839:1877899-1878001 [Klebsiella pneumoniae RYC492]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT232618 WP_001285584.1 NZ_CP091065:c1624519-1624151 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT232618 NZ_CP127839:c1878008-1877863 [Klebsiella pneumoniae RYC492]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References