Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 475912..476530 Replicon chromosome
Accession NZ_CP091065
Organism Escherichia coli strain DOC14

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag L1123_RS02360 Protein ID WP_001291435.1
Coordinates 475912..476130 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag L1123_RS02365 Protein ID WP_000344800.1
Coordinates 476156..476530 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1123_RS02325 (471201) 471201..471773 + 573 WP_000779842.1 YbaY family lipoprotein -
L1123_RS02330 (471804) 471804..472115 - 312 WP_000409911.1 MGMT family protein -
L1123_RS02340 (472494) 472494..472847 + 354 WP_000878140.1 DUF1428 domain-containing protein -
L1123_RS02345 (472889) 472889..474439 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
L1123_RS02350 (474603) 474603..475073 - 471 WP_000136192.1 YlaC family protein -
L1123_RS02355 (475189) 475189..475740 - 552 WP_000102564.1 maltose O-acetyltransferase -
L1123_RS02360 (475912) 475912..476130 - 219 WP_001291435.1 HHA domain-containing protein Toxin
L1123_RS02365 (476156) 476156..476530 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
L1123_RS02370 (477076) 477076..480225 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
L1123_RS02375 (480248) 480248..481441 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T232612 WP_001291435.1 NZ_CP091065:c476130-475912 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T232612 NZ_CP127834:490972-491322 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT232612 WP_000344800.1 NZ_CP091065:c476530-476156 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT232612 NZ_CP127834:490685-490966 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References