Detailed information of TA system TA232607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 156272..156942
• Replicon: plasmid pKP29105-1
• Accession: NZ_CP091062
• Organism: Klebsiella pneumoniae strain KP29105

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: L1062_RS27400
• Protein ID: WP_004213072.1
• Coordinates: 156272..156715 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: L1062_RS27405
• Protein ID: WP_004213073.1
• Coordinates: 156712..156942 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1062_RS27365 (L1062_27365)	151682..151957	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
L1062_RS27370 (L1062_27370)	152020..152511	+	492	WP_011251327.1	DM13 domain-containing protein	-
L1062_RS27375 (L1062_27375)	152560..153480	+	921	WP_232700329.1	DUF1471 domain-containing protein	-
L1062_RS27380 (L1062_27380)	153571..153974	+	404	Protein_167	GAF domain-containing protein	-
L1062_RS27385 (L1062_27385)	154492..155128	-	637	Protein_168	mucoid phenotype regulator RmpA2	-
L1062_RS27390 (L1062_27390)	155545..155849	+	305	Protein_169	transposase	-
L1062_RS27395 (L1062_27395)	155872..156123	-	252	WP_186987481.1	hypothetical protein	-
L1062_RS27400 (L1062_27400)	156272..156715	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L1062_RS27405 (L1062_27405)	156712..156942	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L1062_RS27410 (L1062_27410)	157550..158683	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
L1062_RS27415 (L1062_27415)	158699..158992	+	294	WP_004213076.1	hypothetical protein	-
L1062_RS27420 (L1062_27420)	158982..159188	-	207	WP_004213077.1	hypothetical protein	-
L1062_RS27425 (L1062_27425)	159540..159830	+	291	WP_004213078.1	hypothetical protein	-
L1062_RS27430 (L1062_27430)	159820..160719	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..229582	229582

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T232607 WP_004213072.1 NZ_CP091062:c156715-156272 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T232607 NZ_CP127409:c1891982-1891728 [Rhodanobacter thiooxydans]
ATGCAGCTGATCTTCTCCGATTCGGCCTGGGAGGATTACCTCCACTGGCAGGCCCACGACCGCAAGCTGGTGGCGCGCAT
CAACAAGCTGATCGACGACACCCTGCGCCATCCCCTCGAAGGCATTGGCAAGCCCGAACCTTTGCGGCACGGGCTGGCAG
GTTACTGGTCGCGCCGCATCAACGACGAGCACCGCATGGTGTACAAGCCCACGGCCGACGCGCTGCTGATTGCGCAGCTG
CGGTATCACTATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT232607 WP_004213073.1 NZ_CP091062:c156942-156712 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT232607 NZ_CP127409:c1892242-1891994 [Rhodanobacter thiooxydans]
ATGGACACCATCACCTACACCAAGGCCCGCTCCAGTCTCGCCGAGACGATGGAGCGCGTCTGCGAGGATCATGCGCCGGT
GATCATCACCAAGAAGGACGAGTCCGTGGTGATGATGTCGCTGGCCGACTACCAGTCCATGGAAGAAACCGCGTACCTGC
TGCGTAGCCCCAGGAACGCGCGCCGCCTGCTGGAGTCGATGGCGCAGCTGGAATCCGGTGGCGGTAAACCGCGCAAGCTG
GCCCGCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620