Detailed information of TA system TA232606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 103651..104561
• Replicon: plasmid pKP29105-1
• Accession: NZ_CP091062
• Organism: Klebsiella pneumoniae strain KP29105

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: L1062_RS27100
• Protein ID: WP_004026354.1
• Coordinates: 103651..104121 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: L1062_RS27105
• Protein ID: WP_004026357.1
• Coordinates: 104118..104561 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1062_RS27075 (L1062_27075)	99063..99461	-	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-
L1062_RS27080 (L1062_27080)	99798..100292	-	495	WP_004212794.1	thermonuclease family protein	-
L1062_RS27085 (L1062_27085)	100470..100736	+	267	WP_223175074.1	DUF1173 family protein	-
L1062_RS27090 (L1062_27090)	100769..101419	+	651	WP_068893702.1	DUF1173 family protein	-
L1062_RS27100 (L1062_27100)	103651..104121	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
L1062_RS27105 (L1062_27105)	104118..104561	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
L1062_RS27110 (L1062_27110)	104662..105165	-	504	Protein_113	DUF4113 domain-containing protein	-
L1062_RS27115 (L1062_27115)	105263..105484	-	222	WP_004213643.1	DUF2188 domain-containing protein	-
L1062_RS27120 (L1062_27120)	105665..105784	-	120	Protein_115	Fe3+-citrate ABC transporter substrate-binding protein	-
L1062_RS27125 (L1062_27125)	105859..107985	-	2127	WP_004213640.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
L1062_RS27130 (L1062_27130)	108072..109025	-	954	Protein_117	fec operon regulator FecR	-
L1062_RS27135 (L1062_27135)	109022..109543	-	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..229582	229582

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T232606 WP_004026354.1 NZ_CP091062:c104121-103651 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T232606 NZ_CP127408:c281504-281214 [Stenotrophomonas sp. 59]
ATGGCGATTCTCCAGGCAACAGAAACATTCACGCGATGGCTGCGCGGACTCAACGACAGCAGGGCCCGCGGCGTGATCGT
GGAGCGCCTGCAGCGCGTCGCCCGCGGTCTCGAGGGAGATGTTCGATCAGTGGGTCATGGCGTGAGCGAACTGCGAATCA
ACCATGGGCCGGGATATCGCGTGTATTTCACGCGTCGCGGGAGATCCATCGTCGTGCTCCTGCACGGTGGCGACAAGTCG
AGCCAGCGTCGCGATATCGAAAAAGCGGTCAGGCTCGCGGAGCTGCTGTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT232606 WP_004026357.1 NZ_CP091062:c104561-104118 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT232606 NZ_CP127408:c281175-280882 [Stenotrophomonas sp. 59]
ATGAGCAAGCTGAAGACGATTCCCTTCGATCCCGCCGACTATCTGGCTGATGACGTCGCAATCGCCGCCTATCTGAGCGA
GGCGTTGGCAGCAGGTGATGCGGGCCACTTCCAGGAGGCGCTCGGAACAGTGGCGCGCGCCCGCGGCATGGGCGGCGTTG
CCGAGGTCTCCGGTCTTGGACGCGAGAGCCTGTACAAGGCGCTGCGCCCCGGAGCACACCCACGCTTCGATACCGTGCAA
CGTGTGCTGTCGGCGCTTGGGGTGCACCTGATCGTGCAGGCCGGCAAGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9