Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5438675..5439300 Replicon chromosome
Accession NZ_CP091061
Organism Klebsiella pneumoniae strain KP29105

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag L1062_RS26190 Protein ID WP_012737091.1
Coordinates 5438675..5439058 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L1062_RS26195 Protein ID WP_004150355.1
Coordinates 5439058..5439300 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1062_RS26175 (5436041) 5436041..5436943 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L1062_RS26180 (5436940) 5436940..5437575 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L1062_RS26185 (5437572) 5437572..5438501 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L1062_RS26190 (5438675) 5438675..5439058 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
L1062_RS26195 (5439058) 5439058..5439300 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L1062_RS26200 (5439505) 5439505..5440422 + 918 WP_012737090.1 alpha/beta hydrolase -
L1062_RS26205 (5440436) 5440436..5441377 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L1062_RS26210 (5441422) 5441422..5441859 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L1062_RS26215 (5441856) 5441856..5442716 - 861 WP_004146232.1 virulence factor BrkB family protein -
L1062_RS26220 (5442710) 5442710..5443309 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T232604 WP_012737091.1 NZ_CP091061:c5439058-5438675 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T232604 NZ_CP127406:c117922-116600 [Stenotrophomonas sp. As-14]
ATGGGTACGTTGCGGGTCTGGATGAATGGTGTCGAGGTAGCGCTCTGGGATGATGCGCGGAACCGGGCATCCCGCCTTTC
CTACCTCAGCCAATGGATTGGTTCGACGCAGTCGCGACCGCTGTCCCTTTCGCTCCCCCTGCTGCCCGACGGTGAAAGCC
ATCAAGGTCAGGTGGTCAACGACTACTTCGACAATCTTCTCCCCGATAACGTCACCATCCGCAACCGGATCCGCGACCGG
TTCGCCACGCGCAGCAGCAATACGTTCGATCTGCTTGAAGCCATTGGCCGGGATTGTGTGGGCGCGGTGCAGCTGTTGCC
ACATTCGCGCACACCGGATGCCATCCAGCGGATAACGTTCGAACCATTGAATGACTCAGGCGTCGCTGAAGTCCTGCGCC
AGGTCACAACAAGTGCATTGCCTGGCACTTCTTCCAGCGACGATGCGTTCCGCATTTCCATCGCTGGAGCGCAGGAAAAG
ACCGGCCTGCTGCTGCACGATGGTCAATGGTGCATTCCTACCGGAAGTACGCCGTCCACGCACATCTTCAAGCTGCCGTT
GGGTATCGTCGGCAACATGCGTGCGGACATGCACCAGTCCGTGGAGAACGAGTGGCTGTGCATGCACCTGCTGGCAGCCT
TTGGAATTCCCGTCGCGGATACGCAGATGGGGCGTTTTGAAGATCAAGGTGCACTGATCGTCGAACGTTTCGACCGGCGA
CTGGCTGCGGATCGCGTGTGGTGGCTTCGCTTGCCGCAGGAAGATATGTGTCAGGTCTTTGGCCTCCCCGCTGCCCGGAA
GTACGAATCCGATGGCGGCCCCGGCATCGTGGCCATCATGGATCTGCTTCGGCAATCCGAGCAGCCTGAGGATCGAGCAA
CCTTCTTCAAGACGCAGATCCTGTTCTGGATGCTTGCCGCGACCGACGGCCACGCAAAGAACTTCAGCGTCCACATCGAC
GCGGGTGGGCGTTTCCGTCTGACTCCTGTCTACGACGTGCTGTCGGTATATCCCATCCTCGGCCGGGGGCCCAATCAACT
CGATCCGCACAACGCGCAGCTTGCCATGGCGGTAGAGGGCAAGAACCGCCATCGAAAACTGCATCTTGTGCGACGCCGGC
ACTGGAACGAAACCGCACGTGCCTGTGGCGTAGCCGATGGCGCAGAGCCATGGATCGAAGCGTTGATCGCTTCCGTGGAG
CCCGCCATCGAACACGTCACACGCCAGCTTCCAAGCGGCTTCCCTTCGACCGTGTCCGAACCTGTTTTCAAGGGACTGCG
TGCTGCGGCGACCCGCCTTTCCAGCATGGCCCCCGACGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT232604 WP_004150355.1 NZ_CP091061:c5439300-5439058 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT232604 NZ_CP127406:c118132-117926 [Stenotrophomonas sp. As-14]
ATGCGCGCGTTCAGACGCCAGGCCGGGCTGAGTCAAGCACAGCTTGCCGAGCGACTGGGCATCTCCCGCCAGGCAGTCGG
CGCATTGGAACGCGATCCAGCGTCTGCCAGCTTCGAGCGATTGATGCGAGTCTGGGCCGTGTTGGGCCTGGAAATCAGCC
TGCAGCAGCGAAGTCCCCGGGAAAACACGTCGACTTCGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References