Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4801436..4802246 Replicon chromosome
Accession NZ_CP091061
Organism Klebsiella pneumoniae strain KP29105

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag L1062_RS23215 Protein ID WP_014908042.1
Coordinates 4801436..4801969 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag L1062_RS23220 Protein ID WP_002887278.1
Coordinates 4801980..4802246 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1062_RS23210 (4800267) 4800267..4801388 + 1122 WP_012737335.1 cupin domain-containing protein -
L1062_RS23215 (4801436) 4801436..4801969 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
L1062_RS23220 (4801980) 4801980..4802246 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
L1062_RS23225 (4802349) 4802349..4803782 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
L1062_RS23230 (4803772) 4803772..4804455 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
L1062_RS23235 (4804627) 4804627..4806012 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
L1062_RS23240 (4806030) 4806030..4806374 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T232602 WP_014908042.1 NZ_CP091061:c4801969-4801436 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T232602 NZ_CP127404:c3074679-3073357 [Stenotrophomonas sp. As-6]
ATGGGTACGTTGCGGGTCTGGATGAATGGTGTCGAGGTAGCGCTCTGGGATGATGCGCGGAACCGGGCATCCCGCCTTTC
CTACCTCAGCCAATGGATTGGTTCGACGCAGTCGCGACCGCTGTCCCTTTCGCTCCCCCTGCTGCCCGACGGTGAAAGCC
ATCAAGGTCAGGTGGTCAACGACTACTTCGACAATCTTCTCCCCGATAACGTCACCATCCGCAACCGGATCCGCGACCGG
TTCGCCACGCGCAGCAGCAATACGTTCGATCTGCTTGAAGCCATTGGCCGGGATTGTGTGGGCGCGGTGCAGCTGTTGCC
ACATTCGCGCACACCGGATGCCATCCAGCGGATAACGTTCGAACCATTGAATGACTCAGGCGTCGCTGAAGTCCTGCGCC
AGGTCACAACAAGTGCATTGCCTGGCACTTCTTCCAGCGACGATGCGTTCCGCATTTCCATCGCTGGAGCGCAGGAAAAG
ACCGGCCTGCTGCTGCACGATGGTCAATGGTGCATTCCTACCGGAAGTACGCCGTCCACGCACATCTTCAAGCTGCCGTT
GGGTATCGTCGGCAACATGCGTGCGGACATGCACCAGTCCGTGGAGAACGAGTGGCTGTGCATGCACCTGCTGGCAGCCT
TTGGAATTCCCGTCGCGGATACGCAGATGGGGCGTTTTGAAGATCAAGGTGCACTGATCGTCGAACGTTTCGACCGGCGA
CTGGCTGCGGATCGCGTGTGGTGGCTTCGCTTGCCGCAGGAAGATATGTGTCAGGTCTTTGGCCTCCCCGCTGCCCGGAA
GTACGAATCCGATGGCGGCCCCGGCATCGTGGCCATCATGGATCTGCTTCGGCAATCCGAGCAGCCTGAGGATCGAGCAA
CCTTCTTCAAGACGCAGATCCTGTTCTGGATGCTTGCCGCGACCGACGGCCACGCAAAGAACTTCAGCGTCCACATCGAC
GCGGGTGGGCGTTTCCGTCTGACTCCTGTCTACGACGTGCTGTCGGTATATCCCATCCTCGGCCGGGGGCCCAATCAACT
CGATCCGCACAACGCGCAGCTTGCCATGGCGGTAGAGGGCAAGAACCGCCATCGAAAACTGCATCTTGTGCGACGCCGGC
ACTGGAACGAAACCGCACGTGCCTGTGGCGTAGCCGATGGCGCAGAGCCATGGATCGAAGCGTTGATCGCTTCCGTGGAG
CCCGCCATCGAACACGTCACACGCCAGCTTCCAAGCGGCTTCCCTTCGACCGTGTCCGAACCTGTTTTCAAGGGACTGCG
TGCTGCGGCGACCCGCCTTTCCAGCATGGCCCCCGACGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT232602 WP_002887278.1 NZ_CP091061:c4802246-4801980 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT232602 NZ_CP127404:c3074889-3074683 [Stenotrophomonas sp. As-6]
ATGCGCGCGTTCAGACGCCAGGCCGGGCTGAGTCAAGCACAGCTTGCCGAGCGACTGGGCATCTCCCGCCAGGCAGTCGG
CGCATTGGAACGCGATCCAGCGTCTGCCAGCTTCGAGCGATTGATGCGAGTCTGGGCCGTGTTGGGCCTGGAAATCAGCC
TGCAGCAGCGAAGTCCCCGGGAAAACACGTCGACTTCGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References