Detailed information of TA system TA232587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 157337..158007
• Replicon: plasmid pKP30086-1
• Accession: NZ_CP091059
• Organism: Klebsiella pneumoniae strain KP30086

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: L1063_RS27420
• Protein ID: WP_004213072.1
• Coordinates: 157337..157780 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: L1063_RS27425
• Protein ID: WP_004213073.1
• Coordinates: 157777..158007 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1063_RS27385 (L1063_27385)	152747..153022	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
L1063_RS27390 (L1063_27390)	153085..153576	+	492	WP_011251327.1	DM13 domain-containing protein	-
L1063_RS27395 (L1063_27395)	153625..154545	+	921	WP_232700329.1	DUF1471 domain-containing protein	-
L1063_RS27400 (L1063_27400)	154636..155039	+	404	Protein_169	GAF domain-containing protein	-
L1063_RS27405 (L1063_27405)	155557..156193	-	637	Protein_170	mucoid phenotype regulator RmpA2	-
L1063_RS27410 (L1063_27410)	156610..156914	+	305	Protein_171	transposase	-
L1063_RS27415 (L1063_27415)	156937..157188	-	252	WP_186987481.1	hypothetical protein	-
L1063_RS27420 (L1063_27420)	157337..157780	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L1063_RS27425 (L1063_27425)	157777..158007	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L1063_RS27430 (L1063_27430)	158615..159748	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
L1063_RS27435 (L1063_27435)	159764..160057	+	294	WP_004213076.1	hypothetical protein	-
L1063_RS27440 (L1063_27440)	160047..160253	-	207	WP_004213077.1	hypothetical protein	-
L1063_RS27445 (L1063_27445)	160605..160895	+	291	WP_004213078.1	hypothetical protein	-
L1063_RS27450 (L1063_27450)	160885..161784	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..230647	230647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T232587 WP_004213072.1 NZ_CP091059:c157780-157337 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T232587 NZ_CP127390:826251-826505 [Propionimicrobium sp. PCR01-08-3]
GTGAACCTCTCATGGACCGAAGACGCCTGGAACGACTACCTGTATTGGCAAGAACAGGACAAGAAGACCCTGCGACGTAT
CAACAAGTTGATTTCCGACACGATGCGTCACCCATCGGAAGGAATTGGCAAGCCTGAGCCGTTGAAGTGGGAGATGCAAG
GGGCCTGGTCAAGACGAATCGACACGGCGAATCGGCTCATCTACGTGGTCCTGGACGACACAGTGTGCATCCTTTCCGCC
AAGGACCACTACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT232587 WP_004213073.1 NZ_CP091059:c158007-157777 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT232587 NZ_CP127390:825982-826254 [Propionimicrobium sp. PCR01-08-3]
ATGGTCACAGCAGTCACGTACAGCAACTTCCGCAAGAGTCTCAAGACCTTCATGAGGCAGGTGAACGAGGACGCGGACAT
GCTCCTCGTTACCAATACCGACCCGGTCGACAACGTGGTTGTCATGAGCGCGGCAGACTACGACTCCCTCATGGAGACGG
TACGGATCTACCAAAACCCCTATCTGCAAGACAAGGTTCTCCGCGGGTTGGAGAGCGTCCGCCATGGGCATACCACCGCT
CATGAACTCATTGATGTGGACGACCCTTCGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620