Detailed information of TA system TA232586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 103651..104561
• Replicon: plasmid pKP30086-1
• Accession: NZ_CP091059
• Organism: Klebsiella pneumoniae strain KP30086

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: L1063_RS27110
• Protein ID: WP_004026354.1
• Coordinates: 103651..104121 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: L1063_RS27115
• Protein ID: WP_004026357.1
• Coordinates: 104118..104561 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1063_RS27085 (L1063_27085)	99063..99461	-	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-
L1063_RS27090 (L1063_27090)	99798..100292	-	495	WP_004212794.1	thermonuclease family protein	-
L1063_RS27095 (L1063_27095)	100470..100736	+	267	WP_223175074.1	DUF1173 family protein	-
L1063_RS27100 (L1063_27100)	100769..101419	+	651	WP_068893702.1	DUF1173 family protein	-
L1063_RS27110 (L1063_27110)	103651..104121	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
L1063_RS27115 (L1063_27115)	104118..104561	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
L1063_RS27120 (L1063_27120)	104662..105165	-	504	Protein_113	DUF4113 domain-containing protein	-
L1063_RS27125 (L1063_27125)	105263..105484	-	222	WP_004213643.1	DUF2188 domain-containing protein	-
L1063_RS27130 (L1063_27130)	105665..105784	-	120	Protein_115	Fe3+-citrate ABC transporter substrate-binding protein	-
L1063_RS27135 (L1063_27135)	105859..106677	-	819	Protein_116	TonB-dependent siderophore receptor	-
L1063_RS27140 (L1063_27140)	106710..107678	+	969	WP_135725715.1	IS5 family transposase	-
L1063_RS27145 (L1063_27145)	107735..109051	-	1317	Protein_118	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..230647	230647
	-	inside	IScluster/Tn	-	-	97612..107678	10066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T232586 WP_004026354.1 NZ_CP091059:c104121-103651 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T232586 NZ_CP127390:334901-335161 [Propionimicrobium sp. PCR01-08-3]
GTGAAGTTGGTAATTACGGCCAATGGCTGGAATGACTACTGCCACTGGCAAGAGACTGATCGCGCCACACTCAAAAGAGT
GAACAAATTAATTGACGCTGCGATGCGCACACCGAAGGACGGGATCGGCAAGCCCGAAGCGCTCAAGTATCAGCTAGGTG
AGGTGTGGTCGCGTCGCATCACCGAGGAACATCGCCTTGTTTACAGAATTGGTGATGCTGAGATCATCATCCTCCAAGCT
CGCTTCCATTACGACGCGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT232586 WP_004026357.1 NZ_CP091059:c104561-104118 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT232586 NZ_CP127390:334653-334904 [Propionimicrobium sp. PCR01-08-3]
ATGTCAACGATCTCCGCGAGCACGGCCCGCAGGGAGCTATTTCCGCTCATTGCCGAGGTGAATGAAGATCATACCGAGGT
ACGCATTACCTCAAAGGCCGGGAATGCTGTTCTGATCTCCGAAGCGGAGTTTGAGGCCTGGCAGACAACTCGTTATCTGT
TCTCGACCAAGGCCAATGCGCAGCATCTGCTCGAATCCCTTGCTCAGGCGGCGGCCGGTGATGTGCATAGCCATGACCTT
GACCGCGCGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9