Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5441710..5442335 Replicon chromosome
Accession NZ_CP091058
Organism Klebsiella pneumoniae strain KP30086

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag L1063_RS26200 Protein ID WP_012737091.1
Coordinates 5441710..5442093 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L1063_RS26205 Protein ID WP_004150355.1
Coordinates 5442093..5442335 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1063_RS26185 (5439076) 5439076..5439978 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L1063_RS26190 (5439975) 5439975..5440610 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L1063_RS26195 (5440607) 5440607..5441536 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L1063_RS26200 (5441710) 5441710..5442093 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
L1063_RS26205 (5442093) 5442093..5442335 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L1063_RS26210 (5442540) 5442540..5443457 + 918 WP_012737090.1 alpha/beta hydrolase -
L1063_RS26215 (5443471) 5443471..5444412 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L1063_RS26220 (5444457) 5444457..5444894 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L1063_RS26225 (5444891) 5444891..5445751 - 861 WP_004146232.1 virulence factor BrkB family protein -
L1063_RS26230 (5445745) 5445745..5446344 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T232584 WP_012737091.1 NZ_CP091058:c5442093-5441710 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T232584 NZ_CP127390:c35682-35368 [Propionimicrobium sp. PCR01-08-3]
ATGAGGATACTTCGAACGGAGGTCTACGAGCGGTGGTTCCGCCGTTTGAAGGATGCTCAAGGAAAGGCGCGAATCGACAT
CGCGTTACGCCGCTGCACCCTGGTCGGAAGTGTTGTCGGCGATATCAAGCCGGTAGGTGACGGTGTTCAGGAAATGCGGA
TCCACTCGGGGCCGGGATATCGGGTCTACTTCCTGCACCGTCCGAATGAGCTAATGCTCCTGGTAATCGGCGGAGACAAA
TCCAGTCAGTCCCGAGACATAGAGAAAGCAAAAGCCATCGCGAAACAGCTGAAGGAGGAAGGACAATGGCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT232584 WP_004150355.1 NZ_CP091058:c5442335-5442093 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT232584 NZ_CP127390:c35377-35084 [Propionimicrobium sp. PCR01-08-3]
ATGGCGCTAACGATAGAAGCATGGGACCCGAGCGAGCATCTTGAGACCCAGGATGACGTGGTGGCATATCTGAATGCGGC
ACTTGAAGACGGCGACCCGGCACTCCTGCAAGCTGCACTCGGCGACATCGCGAAGGCTCGGGGAATGAGCAGCATTGCTC
GCGAGGCAGGGGTTGGACGCGAGTCTCTCTACAAGTCATTGCGCATTGACGGCAACCCTTCGTGGCAAACCATCACCAAA
GTCCTCGCCGCCCTCGATATCCGGCTCGAAGCCAAGGCACCGGCTAACGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References