Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4804471..4805281 Replicon chromosome
Accession NZ_CP091058
Organism Klebsiella pneumoniae strain KP30086

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag L1063_RS23225 Protein ID WP_014908042.1
Coordinates 4804471..4805004 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag L1063_RS23230 Protein ID WP_002887278.1
Coordinates 4805015..4805281 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1063_RS23220 (4803302) 4803302..4804423 + 1122 WP_012737335.1 cupin domain-containing protein -
L1063_RS23225 (4804471) 4804471..4805004 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
L1063_RS23230 (4805015) 4805015..4805281 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
L1063_RS23235 (4805384) 4805384..4806817 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
L1063_RS23240 (4806807) 4806807..4807490 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
L1063_RS23245 (4807662) 4807662..4809047 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
L1063_RS23250 (4809065) 4809065..4809409 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T232582 WP_014908042.1 NZ_CP091058:c4805004-4804471 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T232582 NZ_CP127389:c4100533-4099220 [Proteus sp. HZ0627]
ATGGCAAGCCTTGATGTATATATGAATGAATACTATGTGGGCATTTTTACTAAAAAAAGCTCAGGGGCGCATTCATTTCA
ATATGCAGAAGAATGGGTTAACCAACAAGGAAGTCGTCCTATTTCTCTTTCTATGCCACTGCAACTGCAAGAATATCAAG
GTGATTGCGTCTATAATTTCTTTGATAACCTTTTACCTGATAATACTGAAATTAGAAATCGCATTGTGACTCGCTATAAT
GCGAATTCAAACCAACCATTTGATCTATTAACTGCGGTTGGTGCAGATACCGTCGGTGCATTAAGGTTACTTCCTCATGG
AAGTACGCCAGCTAATATCAAAAAAATAGAATATAGCAAACTTAACGAATCTGAACTGGAGAGAATTCTGCTAGGATATC
AATCCAGTACTCCACTTGGTATGCTTGAAGAGTATACTGATTTCAGAATTTCTATCGCTGGCGCGCAAGAAAAAACGGCA
TTACTTTTAAAAGATGATCATTGGTGTGTGCCACACAATAATACGCCGACTACACATATTATTAAGCTGCCCATTGGTGT
TATTGAAGGTCATTCCTATACAATGGATTTATCGAATAGTGTAGAAAATGAATATCTTTGTACCTTAATTGCCAAAGAAT
TTGGTCTCTCTGTTCCTCACTGCTCTATTATTAAAACAAAAAATATTAAAGCGCTTGCTGTAGAACGTTTTGATAGAAAA
TACTCATCAGATAAATCGTGGATAATACGTTTACCCCAAGAAGATTTTTGCCAAATTCTAAATATACCCTCAGCCCTAAA
ATATGAAAACGAAGGCGGGCCAGGTATTATCGATATCATGAGTTATTTACTTGGCTCATCAAATGCCGATCAAGACCGTT
TTCATTTTATGAAGGCACAAGTACTGTTTTGGCTTTTGGCTGCAACAGATGGGCATGCAAAGAATTTTTCTGTTTTTATC
AACCAAGAAGGGCGTTTTCATCTCACTCCTCTTTACGACATTATGTCTATCTATCCCAATTTTGGAGGGAGAGGGCTTAA
TCCACGAGATGCAAAACTCGCAATGGGATTACAAGCAAGCCGTGGTAAAAAATACGCTATAGAGCAAATATTCCCTCGTC
ACTTTTATAAAACTGCCGAAACTGTTGGATTTGAACGTTCATTACTGACTGATATTTTTAATTATTTTTATACAGAAATG
GACAGTGTAATAGAACGTGTAAAACAACAACTTCCTAAAGACTTCCCTACTGAGATTAGCAATACAATATTGAATGGTAT
GAAAGCACGCTCTACACGATTAGCGACTATTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT232582 WP_002887278.1 NZ_CP091058:c4805281-4805015 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT232582 NZ_CP127389:c4100791-4100534 [Proteus sp. HZ0627]
ATGATCATAATTAACGCCTATCAACTCAGCGTGCATCTAAAAGATGTACGATTAACAAAAAAGCTTTCTCAAGGTAAAGT
CGCTCAAAAAGTAGGAATTCGACAAGACACCGTCTCCAATTTTGAACTTAACCCTAATTCCACCAAACTCGAAACCTTTT
TCAAGCTGCTCTCTGCACTGAATTTAGAGATGGAAATTCATCCTAAAGATGCTAAAAATAGTTTAGCACGTAAAAGTGAA
TGGAAAGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References